Crystal structure of Arabidopsis DWARF14-LIKE2 (DLK2) reveals a distinct substrate binding pocket architecture.
Crystal structure of Arabidopsis DWARF14-LIKE2 (DLK2) reveals a distinct substrate binding pocket architecture.
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拟南芥DWARF14-LIKE2(DLK2)的晶体结构显示出明显的底物结合口袋结构。
作者：
在拟南芥中，Sigma因子B调节因子RsbQ类似的α/β水解酶家族包含Strigolacone(SL)受体DWARF14(AtD14)、Karrikin受体Karrikin INSENSITIVE2(AtKAI2)和DWARF14-LIKE2(AtDLK2)，这是一个功能未知的蛋白质。尽管AtD14和AtKAI2的蛋白质折叠非常相似，但它们在配体结合口袋的大小和结构上不同，影响了它们的底物特异性。我们给出了AtDLK2的1.5°晶体结构，揭示了蛋白质家族中最小的配体结合口袋，中间有两个独特的甘氨酸残基。我们在蛋白质的LID结构域中发现了一个守门人残基，并提出了一种吡咯-喹啉-二酮化合物，它能抑制AtDLK2‘S的酶活性。
In Arabidopsis thaliana , the Sigma factor B regulator RsbQ‐like family of α/β hydrolases contains the strigolactone (SL) receptor DWARF14 (AtD14), the karrikin receptor KARRIKIN INSENSITIVE2 (AtKAI2), and DWARF14‐LIKE2 (AtDLK2), a protein of unknown function. Despite very similar protein folds, AtD14 and AtKAI2 differ in size and architecture of their ligand binding pockets, influencing their substrate specificity. We present the 1.5 Å crystal structure of AtDLK2, revealing the smallest ligand binding pocket in the protein family, bordered by two unique glycine residues. We identified a gatekeeper residue in the protein's lid domain and present a pyrrolo‐quinoline‐dione compound that inhibits AtDLK2's enzymatic activity.
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影响因子：
64.8
作者：
Jumper J;Evans R;Pritzel A;Green T;Figurnov M;Ronneberger O;Tunyasuvunakool K;Bates R;Žídek A;Potapenko A;Bridgland A;Meyer C;Kohl SAA;Ballard AJ;Cowie A;Romera-Paredes B;Nikolov S;Jain R;Adler J;Back T;Petersen S;Reiman D;Clancy E;Zielinski M;Steinegger M;Pacholska M;Berghammer T;Bodenstein S;Silver D;Vinyals O;Senior AW;Kavukcuoglu K;Kohli P;Hassabis D
通讯作者：
Hassabis D
影响因子：
8.8
作者：
Burger, Marco;Mashiguchi, Kiyoshi;Chory, Joanne
通讯作者：
Chory, Joanne
影响因子：
9.2
作者：
Hamiaux, Cyril;Drummond, Revel S. M.;Snowden, Kimberley C.
通讯作者：
Snowden, Kimberley C.
影响因子：
9.4
作者：
Ho-Plagaro, Tania;Morcillo, Rafael J. L.;Garcia-Garrido, Jose Manuel
通讯作者：
Garcia-Garrido, Jose Manuel
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH