The complete genome sequence of Hafnia alvei A23BA; a potential antibiotic-producing rhizobacterium.
The complete genome sequence of Hafnia alvei A23BA; a potential antibiotic-producing rhizobacterium.
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DOI：
10.1186/s13104-020-05418-2
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发表时间：
2021-01-06
影响因子：
1.8
通讯作者：
Lamb AJ
中科院分区：
文献类型：
--
作者：
Awolope OK;O'Driscoll NH;Di Salvo A;Lamb AJ
对新型抗生素的迫切需要怎么强调都不过分。从植物根际分离出蜂房哈夫尼菌A23 BA，作为从土壤样品中回收新型产芽孢杆菌菌株的努力的一部分。对分离株的基因组进行测序，以便于挖掘潜在的编码抗生素的生物合成基因簇，并深入了解这些基因簇是如何被激活的。在此，我们报道了H. alvei A23 BA从Illumina HiSeq和GridION读数的混合组装中获得。基因组由一条环状染色体和一个环状质粒组成，大小为4.77 Mb，GC含量为48.77%。组装完成率为99.5%，具有包括4，217个CDS、125个RNA和30个假基因的基因组特征。还鉴定了硫肽、β-内酯、铁载体和高丝氨酸内酯生物合成基因簇。其他感兴趣的基因簇包括那些与生物修复，生物控制和植物生长促进相关的基因簇-所有这些都是H。第一次在alvei。该数据集有助于加快探索物种的生物合成和代谢潜力。此外，该数据集是第一个公布的土壤分离物的基因组序列，丰富了H.肺泡菌株。
The urgent need for novel antibiotics cannot be overemphasized. Hafnia alvei A23BA was isolated from plant rhizosphere as part of an effort to recover novel antibiotic-producing bacterial strains from soil samples. The genome of the isolate was sequenced to facilitate mining for potential antibiotic-encoding biosynthetic gene clusters and to gain insights into how these gene clusters could be activated. Here, we report the complete genome sequence of H. alvei A23BA obtained from the hybrid assembly of Illumina HiSeq and GridION reads. The genome, consisting of a circular chromosome and a circular plasmid, is 4.77 Mb in size with a GC content of 48.77%. The assembly is 99.5% complete with genomic features including 4,217 CDSs, 125 RNAs, and 30 pseudogenes. Thiopeptide, beta-lactone, siderophore, and homoserine lactone biosynthetic gene clusters were also identified. Other gene clusters of interest include those associated with bioremediation, biocontrol, and plant growth promotion- all of which are reported for H. alvei for the first time. This dataset serves to expedite the exploration of the biosynthetic and metabolic potentials of the species. Furthermore, being the first published genome sequence of a soil isolate, this dataset enriches the comparative genomics study of H. alvei strains.
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影响因子：
10.7
作者：
Huerta-Cepas J;Forslund K;Coelho LP;Szklarczyk D;Jensen LJ;von Mering C;Bork P
通讯作者：
Bork P
影响因子：
5.8
作者：
Simao, Felipe A.;Waterhouse, Robert M.;Zdobnov, Evgeny M.
通讯作者：
Zdobnov, Evgeny M.
影响因子：
4.3
作者：
Wick, Ryan R.;Judd, Louise M.;Holt, Kathryn E.
通讯作者：
Holt, Kathryn E.
DOI：
10.1128/genomea.00439-16
发表时间：
2016-06-09
期刊：
Genome announcements
影响因子：
--
作者：
Tian B;Moran NA
通讯作者：
Moran NA
影响因子：
11.8
作者：
Gunthard, H;Pennekamp, A
通讯作者：
Pennekamp, A