First record of Borrelia burgdorferi B31 strain in Dermacentor nitens ticks in the northern region of Parana (Brazil).
First record of Borrelia burgdorferi B31 strain in Dermacentor nitens ticks in the northern region of Parana (Brazil).
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DOI：
10.1590/s1517-83822013000300035
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Vieira ML
中科院分区：
文献类型：
--
作者：
Gonçalves DD;Carreira T;Nunes M;Benitez A;Lopes-Mori FM;Vidotto O;de Freitas JC;Vieira ML
本研究的目的是调查存在的伯氏疏螺旋体广义（s.l.）在巴西巴拉那州Jataizinho农村，以马为饲料的蜱虫中，马用于动物牵引。2008年2月至6月期间，共收集了224只蜱，其中75%被鉴定为光亮革蜱，25%被鉴定为卡亨钝眼蜱。为了放大B。布格多费里公司以5S（rrf）23 S（rrl）rRNA基因间的基因间隔区（ISR）DNA作为巢式PCR的靶基因。两个滴答的D。黑刺鼠种B阳性。布格多费里公司结果表明，两个种均扩增出184 bp的片段，但测序结果显示与B的同源性为99.9%。严格意义上的布尔格多菲菌株B31。这些结果首次表明，在上述农村城市中，存在感染寄生于用于动物牵引的马的蜱的螺旋体DNA。总之，本研究为确定B的感染率开辟了有希望的前景。狭义伯氏疏基因种或马种群中的其他物种，以及巴拉那州感染率对莱姆病的影响。
The aim of this study was to investigate the presence of DNA of Borrelia burgdorferi sensu lato (s.l.) in ticks that feed on horses used for animal traction in rural Jataizinho, Parana, Brazil. Between February and June 2008, a total of 224 ticks was collected of which 75% were identified as Dermacentor nitens and 25% as Amblyomma cajenense. To amplify B. burgdorferi s.l. DNA, the intergenic space region (ISR) between the 5S (rrf) 23S (rrl) rRNA genes was used as targets for nested-PCR. Two ticks of the D. nitens species were positive for B. burgdorferi s.l. Both species showed a fragment of 184 bp, but the sequencing revealed 99.9% homology with the B. burgdorferi sensu stricto (s.s.) strain B31. These results showed, for the first time, the presence of spirochete DNA infecting ticks that parasitize horses used for animal traction, in the rural municipality mentioned. In conclusion, this study opens up promising prospects for determining the infection rate of B. burgdorferi s.s. genospecies or other species in the equine population, as well as the impact of the infection rate on Lyme disease in the state of Parana.
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影响因子：
1.3
作者：
Basso, W;Venturini, L;Dubey, JP
通讯作者：
Dubey, JP
DOI：
10.1016/s1433-1128(04)80016-0
发表时间：
2004-04-01
影响因子：
4.1
作者：
Baptista, S;Quaresma, A;Collares-Pereira, M
通讯作者：
Collares-Pereira, M
DOI：
10.1590/s0037-86821999000600002
发表时间：
1999-11-01
影响因子：
2
作者：
Figueiredo, Luiz Tadeu Moraes;Badra, Soraya Jabur;Szabo, Matias Pablo Juan
通讯作者：
Szabo, Matias Pablo Juan
影响因子：
1.5
作者：
Gaumond, Gwenn;Tyropolis, Allison;Bushmich, Sandra
通讯作者：
Bushmich, Sandra
影响因子：
1.7
作者：
Fonseca, Adivaldo Henrique da;Salles, Roberto de Souza;Yoshinari, Natalino Hajime
通讯作者：
Yoshinari, Natalino Hajime