A Novel Multivariate Index for Pancreatic Cancer Detection Based On the Plasma Free Amino Acid Profile.
A Novel Multivariate Index for Pancreatic Cancer Detection Based On the Plasma Free Amino Acid Profile.
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DOI：
10.1371/journal.pone.0132223
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Katayama K
中科院分区：
文献类型：
--
作者：
Fukutake N;Ueno M;Hiraoka N;Shimada K;Shiraishi K;Saruki N;Ito T;Yamakado M;Ono N;Imaizumi A;Kikuchi S;Yamamoto H;Katayama K
胰腺癌（PC）的发病率在世界范围内持续增加，而大多数患者被诊断为晚期，生存期<12个月。这种不良预后归因于早期检测的困难。在这里，我们开发和评估的多元指数组成的血浆游离氨基酸（PFAA）的PC的早期检测。我们在日本的多个机构进行了一项横断面研究。收集了来自PC患者（n = 360）、慢性胰腺炎（CP）患者（n = 28）和健康对照（HC）受试者（n = 8372）的空腹血浆样本，这些受试者没有明显的癌症，正在接受全面的医学检查。采用液相色谱-质谱联用法测定了19种全氟辛酸的浓度。我们生成了由以下六种PFAA组成的指数：丝氨酸、天冬酰胺、异亮氨酸、丙氨酸、组氨酸和色氨酸作为训练集（120 PC和匹配的600 HC）中的鉴别变量和验证集（240 PC、28 CP和7772 HC）中的评价变量。血浆中的几种氨基酸浓度在PC中显著改变。血浆色氨酸和组氨酸浓度在PC中特别低，而丝氨酸特别高于HC。在训练集中，基于所得指标的受试者工作特征（ROC）曲线分析的曲线下面积（AUC）为0.89 [95%置信区间（CI），0.86-0.93]，以区分PC和HC。在验证集中，所有PC患者与HC受试者相比，基于PFAA指数ROC曲线分析的AUC为0.86（95% CI，0.84-0.89），IIA至IIB期PC患者与HC受试者相比为0.81（95% CI，0.75-0.86），所有PC患者与CP患者相比为0.87（95% CI，0.80-0.93）。这些结果表明PC的PFAA谱与HC的PFAA谱显著不同。PFAA指数是一种很有前途的生物标志物，用于PC的筛查和诊断。
The incidence of pancreatic cancer (PC) continues to increase in the world, while most patients are diagnosed with advanced stages and survive <12 months. This poor prognosis is attributable to difficulty of early detection. Here we developed and evaluated a multivariate index composed of plasma free amino acids (PFAAs) for early detection of PC. We conducted a cross-sectional study in multi-institutions in Japan. Fasting plasma samples from PC patients (n = 360), chronic pancreatitis (CP) patients (n = 28), and healthy control (HC) subjects (n = 8372) without apparent cancers who were undergoing comprehensive medical examinations were collected. Concentrations of 19 PFAAs were measured by liquid chromatography–mass spectrometry. We generated an index consisting of the following six PFAAs: serine, asparagine, isoleucine, alanine, histidine, and tryptophan as variables for discrimination in a training set (120 PC and matching 600 HC) and evaluation in a validation set (240 PC, 28 CP, and 7772 HC). Several amino acid concentrations in plasma were significantly altered in PC. Plasma tryptophan and histidine concentrations in PC were particularly low, while serine was particularly higher than that of HC. The area under curve (AUC) based on receiver operating characteristic (ROC) curve analysis of the resulting index to discriminate PC from HC were 0.89 [95% confidence interval (CI), 0.86–0.93] in the training set. In the validation set, AUCs based on ROC curve analysis of the PFAA index were 0.86 (95% CI, 0.84–0.89) for all PC patients versus HC subjects, 0.81 (95% CI, 0.75–0.86) for PC patients from stage IIA to IIB versus HC subjects, and 0.87 (95% CI, 0.80–0.93) for all PC patients versus CP patients. These findings suggest that the PFAA profile of PC was significantly different from that of HC. The PFAA index is a promising biomarker for screening and diagnosis of PC.
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影响因子：
3.8
作者：
Borgan E;Sitter B;Lingjærde OC;Johnsen H;Lundgren S;Bathen TF;Sørlie T;Børresen-Dale AL;Gribbestad IS
通讯作者：
Gribbestad IS
影响因子：
3.7
作者：
Miyagi Y;Higashiyama M;Gochi A;Akaike M;Ishikawa T;Miura T;Saruki N;Bando E;Kimura H;Imamura F;Moriyama M;Ikeda I;Chiba A;Oshita F;Imaizumi A;Yamamoto H;Miyano H;Horimoto K;Tochikubo O;Mitsushima T;Yamakado M;Okamoto N
通讯作者：
Okamoto N
影响因子：
37.8
作者：
Cheng S;Rhee EP;Larson MG;Lewis GD;McCabe EL;Shen D;Palma MJ;Roberts LD;Dejam A;Souza AL;Deik AA;Magnusson M;Fox CS;O'Donnell CJ;Vasan RS;Melander O;Clish CB;Gerszten RE;Wang TJ
通讯作者：
Wang TJ
DOI：
10.1097/00075197-199901000-00009
发表时间：
1999-01-01
影响因子：
3.1
作者：
Holm, E;Sedlaczek, O;Grips, E
通讯作者：
Grips, E
影响因子：
3.3
作者：
Ihata, Yutaka;Miyagi, Etsuko;Hirahara, Fumiki
通讯作者：
Hirahara, Fumiki