Biocompatibility and Alkaline Phosphatase Activity of Phosphorylated Chitooligosaccharides on the Osteosarcoma MG63 Cell Line.
Biocompatibility and Alkaline Phosphatase Activity of Phosphorylated Chitooligosaccharides on the Osteosarcoma MG63 Cell Line.
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DOI：
10.3390/jfb1010003
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发表时间：
2010-10-22
影响因子：
4.8
通讯作者：
Kim SK
中科院分区：
文献类型：
--
作者：
Venkatesan J;Pangestuti R;Qian ZJ;Ryu B;Kim SK
以H3 PO 4、P2 O 5、Et 3 PO 4和正己醇为溶剂体系制备了磷酸化壳寡糖（P-COS）。采用傅里叶变换红外光谱（FT-IR）、热重-差热分析（TG-DTA）、13 C NMR、31 P NMR、X射线衍射分析、溶解性研究、生物相容性和碱性磷酸酶活性（ALP）等方法对P-COS进行了表征。结果表明，磷酸化发生在OH基团的C3和C6位以及NH 2基团的C2位。FT-IR证实，即使在酸性条件下加热和处理，P-COS中的吡喃糖环也没有分解。通过X射线衍射分析证实了P-COS的非晶态性质。此外，检测了不同浓度的P-COS对骨肉瘤MG 63细胞系的生物相容性和碱性磷酸酶活性，未观察到细胞毒性。培养12 h和24 h后，P-COS的ALP活性均高于对照组。这些结果表明，P-COS是一种生物相容性材料，在未来，P-COS可以为人类开辟许多有前途的药物和临床应用。
Phosphorylated chitooligosaccharides (P-COS) were prepared using a H3PO4, P2O5, Et3PO4 and hexanol solvent system. The P-COS were characterized by Fourier Transform Infrared Spectroscopy (FT-IR), Thermo gravimetric-Differential Thermal Analyzer (TG-DTA), 13C NMR, 31P NMR, X-ray diffraction analysis, solubility studies, biocompatibility and Alkaline Phosphatase Activity (ALP). The results reveal that phosphorylation occurred at the C3 and C6 position of OH groups and the C2 position of NH2 group. FT-IR confirmed no decomposition in pyranose ring in P-COS even with heating and treatment in acidic conditions. The amorphous nature of P-COS was confirmed by X-ray diffraction analysis. Further, the biocompatibility and alkaline phosphatase activity of P-COS were checked against the osteosarcoma MG63 cell line at different concentrations and no cytotoxicity was observed. After 12 h and 24 h of incubation, the ALP activity of P-COS was higher compared with the control group. These results suggest that P-COS is a biocompatible material and in future P-COS could open up a number of promising pharmaceutical and clinical applications to mankind.
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