Effect of water-ageing on dentine bond strength and anti-biofilm activity of bonding agent containing new monomer dimethylaminododecyl methacrylate.
Effect of water-ageing on dentine bond strength and anti-biofilm activity of bonding agent containing new monomer dimethylaminododecyl methacrylate.
复制标题
DOI：
10.1016/j.jdent.2013.03.011
复制
发表时间：
2013-06
影响因子：
4.4
通讯作者：
Xu HH
中科院分区：
文献类型：
--
作者：
Zhang K;Cheng L;Wu EJ;Weir MD;Bai Y;Xu HH
本研究旨在研制一种新型抗菌单体甲基丙烯酸二甲氨基十二烷基酯（DMADDM）、纳米银（NAg）和纳米无定形磷酸钙（NACP）的粘接剂，并首次研究水老化6个月对牙本质粘接强度和抗生物膜性能的影响。测试了四种粘合剂：Scotchbond多功能（SBMP）底漆和粘合剂对照; SBMP +5% DMADDM; SBMP + 5% DMADDM + 0.1% NAg;以及SBMP + 5% DMADDM + 0.1% NAg，粘合剂中含有20% NACP。将样品在37 °C下水老化1天和6个月。然后测量牙本质剪切粘结强度。牙菌斑微宇宙生物膜模型被用于在水老化的标本上培养细菌，并测量代谢活性、菌落形成单位（CFU）和乳酸产生。对于SBMP对照，牙本质粘结强度在6个月的水老化中显示出35%的损失（平均值±标准差; n = 10）;相比之下，新的抗菌粘结剂没有显示出强度损失。含有DMADDM-NAg-NACP的粘合剂通过大大降低生物膜活力、代谢活性和酸产生而赋予强的抗菌效果。与SBMP对照相比，生物膜CFU减少了超过两个数量级。此外，DMADDM-NAg-NACP粘合剂表现出长期的抗菌性能，在1 d和6个月之间没有显著差异（p > 0.1）。将DMADDM-NAg-NACP在粘合剂中进行分散，产生了有效和持久的抗菌性能，并且在6个月的水老化后比商业对照具有更强的粘合强度。新型抗菌粘接剂有望抑制边缘生物膜和龋齿。DMADDM-NAg-NACP的掺入方法对其它胶粘剂、水泥和复合材料具有广泛的适用性。
The objectives of this study were to develop bonding agent containing a new antibacterial monomer dimethylaminododecyl methacrylate (DMADDM) as well as nanoparticles of silver (NAg) and nanoparticles of amorphous calcium phosphate (NACP), and to investigate the effects of water-ageing for 6 months on dentine bond strength and anti-biofilm properties for the first time. Four bonding agents were tested: Scotchbond Multi-Purpose (SBMP) Primer and Adhesive control; SBMP + 5% DMADDM; SBMP + 5% DMADDM + 0.1% NAg; and SBMP + 5% DMADDM + 0.1% NAg with 20% NACP in adhesive. Specimens were water-aged for 1 d and 6 months at 37 °C. Then the dentine shear bond strengths were measured. A dental plaque microcosm biofilm model was used to inoculate bacteria on water-aged specimens and to measure metabolic activity, colony-forming units (CFUs), and lactic acid production. Dentine bond strength showed a 35% loss in 6 months of water-ageing for SBMP control (mean ± sd; n = 10); in contrast, the new antibacterial bonding agents showed no strength loss. The DMADDM–NAg–NACP containing bonding agent imparted a strong antibacterial effect by greatly reducing biofilm viability, metabolic activity and acid production. The biofilm CFU was reduced by more than two orders of magnitude, compared to SBMP control. Furthermore, the DMADDM–NAg–NACP bonding agent exhibited a long-term antibacterial performance, with no significant difference between 1 d and 6 months (p > 0.1). Incorporating DMADDM–NAg–NACP in bonding agent yielded potent and long-lasting antibacterial properties, and much stronger bond strength after 6 months of water-ageing than a commercial control. The new antibacterial bonding agent is promising to inhibit biofilms and caries at the margins. The method of DMADDM–NAg–NACP incorporation may have a wide applicability to other adhesives, cements and composites.
登录
查看更多内容
影响因子：
3.3
作者：
Chrysanthakopoulos, Nikolaos A.
通讯作者：
Chrysanthakopoulos, Nikolaos A.
影响因子：
5
作者：
Imazato, S;Kuramoto, A;Peters, MC
通讯作者：
Peters, MC
影响因子：
4.5
作者：
Damm, Cornelia;Muensted, Helmut;Roesch, Alfons
通讯作者：
Roesch, Alfons
DOI：
10.14219/jada.archive.2012.0238
发表时间：
2012-06-01
影响因子：
3.9
作者：
Gordan, Valeria V.;Riley, Joseph L., III;Gilbert, Gregg H.
通讯作者：
Gilbert, Gregg H.
影响因子：
5
作者：
Breschi, Lorenzo;Mazzoni, Annalisa;Nato, Fernando;Carrilho, Marcela;Visintini, Erika;Tjaderhane, Leo;Ruggeri, Alessandra, Jr.;Tay, Franklin R.;Dorigo, Elettra De Stefano;Pashley, David H.
通讯作者：
Pashley, David H.