Integrative Analysis of miRNA and mRNA Expression Profiles in Mammary Glands of Holstein Cows Artificially Infected with Staphylococcus aureus.
Integrative Analysis of miRNA and mRNA Expression Profiles in Mammary Glands of Holstein Cows Artificially Infected with Staphylococcus aureus.
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DOI：
10.3390/pathogens10050506
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发表时间：
2021-04-22
期刊：
影响因子：
--
通讯作者：
Yang Y
中科院分区：
文献类型：
--
作者：
Wang X;Fan Y;He Y;Han Z;Gong Z;Peng Y;Meng Y;Mao Y;Yang Z;Yang Y
金黄色葡萄球菌引起的乳房炎是乳品行业最棘手的问题之一，它导致全球范围内牛奶产量的损失和奶牛的过早屠宰。很少有研究使用基于miRNA和mRNA表达谱综合分析的综合方法来探索金黄色葡萄球菌引起的奶牛乳房炎的分子机制。本研究将金黄色葡萄球菌(A1、B1和C1)和无菌磷酸盐缓冲液(PBS)(A2、B2和C2)分别注入三头奶牛的不同乳房，利用转录组测序和基因芯片检测了攻毒组和对照组乳腺中miRNA和基因的表达。共鉴定出77个差异表达的microRNAs(DE MiRNAs)和1625个差异表达基因(Deg)。基因本体论(GO)注释和京都基因和基因组百科全书(KEGG)途径分析表明，多个DEG在与免疫和炎症相关的重要术语和途径中得到丰富。DE miRNAs与DEG的整合分析证明，miR-664b、miR-23b-3p、miR-331-5p、miR-19b和miR-2431-3p是调节金黄色葡萄球菌引起的牛乳房炎中CD14分子(CD14)、G蛋白亚基伽玛2(GNG2)、白介素17A(IL17A)、IV型胶原α1链(COL4A1)、微管相关蛋白RP/EB家族成员2(MAPRE2)、NFKB信号调节因子(LDOC1)、低密度脂蛋白受体(LDLR)和S100钙结合蛋白A9(S100A9)表达水平的潜在因子。这些发现有助于了解牛乳腺对金黄色葡萄球菌感染的潜在免疫应答，并为基于miRNA-mRNA的遗传调控网络在耐金黄色葡萄球菌种牛中的应用奠定了基础。
Staphylococcus aureus- induced mastitis is one of the most intractable problems for the dairy industry, which causes loss of milk yield and early slaughter of cows worldwide. Few studies have used a comprehensive approach based on the integrative analysis of miRNA and mRNA expression profiles to explore molecular mechanism in bovine mastitis caused by S. aureus. In this study, S. aureus (A1, B1 and C1) and sterile phosphate buffered saline (PBS) (A2, B2 and C2) were introduced to different udder quarters of three individual cows, and transcriptome sequencing and microarrays were utilized to detected miRNA and gene expression in mammary glands from the challenged and control groups. A total of 77 differentially expressed microRNAs (DE miRNAs) and 1625 differentially expressed genes (DEGs) were identified. Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that multiple DEGs were enriched in significant terms and pathways associated with immunity and inflammation. Integrative analysis between DE miRNAs and DEGs proved that miR-664b, miR-23b-3p, miR-331-5p, miR-19b and miR-2431-3p were potential factors regulating the expression levels of CD14 Molecule (CD14), G protein subunit gamma 2 (GNG2), interleukin 17A (IL17A), collagen type IV alpha 1 chain (COL4A1), microtubule associated protein RP/EB family member 2 (MAPRE2), member of RAS oncogene family (RAP1B), LDOC1 regulator of NFKB signaling (LDOC1), low-density lipoprotein receptor (LDLR) and S100 calcium binding protein A9 (S100A9) in bovine mastitis caused by S. aureus. These findings could enhance the understanding of the underlying immune response in bovine mammary glands against S. aureus infection and provide a useful foundation for future application of the miRNA–mRNA-based genetic regulatory network in the breeding cows resistant to S. aureus.
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影响因子：
4.4
作者：
Gilbert FB;Cunha P;Jensen K;Glass EJ;Foucras G;Robert-Granié C;Rupp R;Rainard P
通讯作者：
Rainard P
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影响因子：
4.4
作者：
Huang J;Luo G;Zhang Z;Wang X;Ju Z;Qi C;Zhang Y;Wang C;Li R;Li J;Yin W;Xu Y;Moisá SJ;Loor JJ;Zhong J
通讯作者：
Zhong J
影响因子：
3.5
作者：
Griesbeck-Zilch, B.;Meyer, H. H. D.;Wellnitz, O.
通讯作者：
Wellnitz, O.
影响因子：
2.7
作者：
He，Jinshui;Kang，Yueya;Ye，Xiaoling
通讯作者：
Ye，Xiaoling
Solexa 测序和定制 microRNA 芯片揭示了患有自然传染性乳腺炎的牛乳腺中的 microRNA 库
影响因子：
2.4
作者：
Ju, Zhihua;Jiang, Qiang;Huang, Jinming
通讯作者：
Huang, Jinming