iTRAQ-proteomics and bioinformatics analyses of mammary tissue from cows with clinical mastitis due to natural infection with Staphylococci aureus.
iTRAQ-proteomics and bioinformatics analyses of mammary tissue from cows with clinical mastitis due to natural infection with Staphylococci aureus.
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对因自然感染金黄色葡萄球菌而患有临床乳腺炎的奶牛的乳腺组织进行 iTRAQ 蛋白质组学和生物信息学分析。
DOI：
10.1186/1471-2164-15-839
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发表时间：
2014-10-02
期刊：
影响因子：
4.4
通讯作者：
Zhong J
中科院分区：
文献类型：
--
作者：
Huang J;Luo G;Zhang Z;Wang X;Ju Z;Qi C;Zhang Y;Wang C;Li R;Li J;Yin W;Xu Y;Moisá SJ;Loor JJ;Zhong J
蛋白质组学和生物信息学可以帮助我们更好地了解发生在牛乳腺炎的生物适应。该系统方法还可以帮助识别用于监测临床和亚临床乳腺炎的生物标志物。本研究的目的是使用同量异位素标签的相对和绝对定量（iTRAQ）筛选潜在的蛋白质与乳腺炎在感染后期阶段。采用iTRAQ结合二维液相色谱-串联质谱（2D-LC-MS/MS）技术对健康奶牛和乳腺炎奶牛的乳腺组织进行分析。通过基因本体论、代谢途径、转录调控网络、动态影响方法和不确定性途径分析对差异表达蛋白进行生物信息学分析。在5%的错误发现率下，从6，499个肽中鉴定出总共768个蛋白质，其与15，879个光谱匹配。与健康乳腺组织相比，36种蛋白质在金黄色葡萄球菌自然感染引起的乳腺炎中显著上调（>1.5倍），而19种蛋白质在金黄色葡萄球菌自然感染引起的乳腺炎中显著下调（<0.67倍）。通过Western印迹和免疫组织化学证实了乳腺炎感染组织中I型胶原α 1（COL 1A 1）和α间（球蛋白）抑制剂H4（ITIH 4）的上调。这篇论文是第一个显示的蛋白质表达的后期反应的乳腺炎病原体，从而揭示了机制与宿主组织损伤。生物信息学分析强调了乳腺炎对胶原蛋白、纤维蛋白原、纤连蛋白、酪蛋白α和硫酸乙酰肝素蛋白聚糖2等蛋白质的影响。我们的发现为进一步研究乳腺炎易感性的候选基因提供了新的线索。COL 1A 1和ITIH 4在乳腺炎乳腺中的上调表达可能与感染后期的组织损伤和修复有关。本文的在线版本（doi：10.1186/1471-2164-15-839）包含补充材料，可供授权用户使用。
Proteomics and bioinformatics may help us better understand the biological adaptations occurring during bovine mastitis. This systems approach also could help identify biomarkers for monitoring clinical and subclinical mastitis. The aim of the present study was to use isobaric tags for relative and absolute quantification (iTRAQ) to screen potential proteins associated with mastitis at late infectious stage. Healthy and mastitic cows’ mammary gland tissues were analyzed using iTRAQ combined with two-dimensional liquid chromatography-tandem mass spectrometry (2D-LC-MS/MS). Bioinformatics analyses of differentially expressed proteins were performed by means of Gene Ontology, metabolic pathways, transcriptional regulation networks using Blast2GO software, the Dynamic Impact Approach and Ingenuity Pathway Analysis. At a false discovery rate of 5%, a total of 768 proteins were identified from 6,499 peptides, which were matched with 15,879 spectra. Compared with healthy mammary gland tissue, 36 proteins were significantly up-regulated (>1.5-fold) while 19 were significantly down-regulated (<0.67-fold) in response to mastitis due to natural infections with Staphylococci aureus. Up-regulation of collagen, type I, alpha 1 (COL1A1) and inter-alpha (Globulin) inhibitor H4 (ITIH4) in the mastitis-infected tissue was confirmed by Western blotting and Immunohistochemistry. This paper is the first to show the protein expression in the late response to a mastitic pathogen, thus, revealing mechanisms associated with host tissue damage. The bioinformatics analyses highlighted the effects of mastitis on proteins such as collagen, fibrinogen, fibronectin, casein alpha and heparan sulfate proteoglycan 2. Our findings provide additional clues for further studies of candidate genes for mastitis susceptibility. The up-regulated expression of COL1A1 and ITIH4 in the mastitic mammary gland may be associated with tissue damage and repair during late stages of infection. The online version of this article (doi:10.1186/1471-2164-15-839) contains supplementary material, which is available to authorized users.
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影响因子：
4.4
作者：
Gilbert FB;Cunha P;Jensen K;Glass EJ;Foucras G;Robert-Granié C;Rupp R;Rainard P
通讯作者：
Rainard P
影响因子：
2.6
作者：
Lutzow YC;Donaldson L;Gray CP;Vuocolo T;Pearson RD;Reverter A;Byrne KA;Sheehy PA;Windon R;Tellam RL
通讯作者：
Tellam RL
影响因子：
15.9
作者：
CHEUNG, AL;KRISHNAN, M;FISCHETTI, VA
通讯作者：
FISCHETTI, VA
影响因子：
3.6
作者：
Fitzgerald, JR;Loughman, A;Foster, TJ
通讯作者：
Foster, TJ
影响因子：
2.6
作者：
Kim Y;Atalla H;Mallard B;Robert C;Karrow N
通讯作者：
Karrow N