Near-infrared squaraine dyes for fluorescence enhanced surface assay.
Near-infrared squaraine dyes for fluorescence enhanced surface assay.
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DOI：
10.1016/j.dyepig.2008.04.008
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发表时间：
2009-01-01
影响因子：
4.5
通讯作者：
Gryczynski, Ignacy
中科院分区：
文献类型：
--
作者：
Matveeva, Evgenia G.;Terpetschnig, Ewald A.;Stevens, Megan;Patsenker, Leonid;Kolosova, Olga S.;Gryczynski, Zygmunt;Gryczynski, Ignacy
关键词：
市售的近红外荧光方碱染料（Seta-635和Seta-670）与抗体共价结合，并采用表面增强免疫测定。从对涂有银纳米颗粒的表面和未涂有银纳米颗粒的玻璃表面测定的荧光强度和寿命变化来看，两种标记的化合物在涂有银的表面上的荧光强度比在未涂有银的表面上的荧光强度增强了15至20倍。此外，在镀银表面的情况下，两种标记的荧光寿命都发生了巨大变化。这两种染料获得的荧光信号增强比先前记录的Rhodamine Red-X和AlexaFluor-647标签更大。
Commercially available, near-infrared fluorescent squaraine dyes (Seta-635 and Seta-670) were covalently bound to antibodies and employed insurface enhanced immunoassay. From fluorescence intensity and lifetime changes determined for a surface which had been coated with silver nanoparticles as well as a non-coated glass surface, both labelled compounds exhibited a 15 to 20-fold enhancement of fluorescence on the silver coated surface compared to that achieved on the non-coated surface. In addition, the fluorescence lifetime changes drastically for both labels in the case of silver-coated surfaces. The fluorescence signal enhancement obtained for the two dyes was greater than that previously recorded for Rhodamine Red-X and AlexaFluor-647 labels.
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DOI：
10.1385/0-89603-308-2:205
发表时间：
1995-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Haugland, R P
通讯作者：
Haugland, R P
影响因子：
4.7
作者：
Gruber, HJ;Hahn, CD;Knaus, HG
通讯作者：
Knaus, HG
DOI：
10.1002/cyto.990100104
发表时间：
1989-01-01
期刊：
CYTOMETRY
影响因子：
--
作者：
MUJUMDAR, RB;ERNST, LA;WAGGONER, AS
通讯作者：
WAGGONER, AS
DOI：
10.1002/cyto.990110313
发表时间：
1990-01-01
期刊：
CYTOMETRY
影响因子：
--
作者：
SOUTHWICK, PL;ERNST, LA;WAGGONER, AS
通讯作者：
WAGGONER, AS
影响因子：
2.9
作者：
Matveeva, E;Gryczynski, Z;Lakowicz, JR
通讯作者：
Lakowicz, JR