AutoRG: An automatized reach-to-grasp platform technology for assessing forelimb motor function, neural circuit activation, and cognition in rodents.
AutoRG: An automatized reach-to-grasp platform technology for assessing forelimb motor function, neural circuit activation, and cognition in rodents.
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DOI：
10.1016/j.jneumeth.2023.109798
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发表时间：
2023-03-01
影响因子：
3
通讯作者：
Karumbaiah, Lohitash
中科院分区：
文献类型：
--
作者：
Forghani, Rameen;Goodnight, Braxton;Latchoumane, Charles-Francois Vincent;Karumbaiah, Lohitash
啮齿动物够握功能评估是评估神经功能损伤和恢复反应的一个翻译功能强大的模型。现有的评估平台依赖于实验人员，成本高昂，或者产量低，产出措施有限。此外，神经激活的直接组织学比较从未在任何新的自动化平台和成熟的单颗粒技术到达任务（SRT）之间进行过。为了解决这些技术和知识差距，我们设计了一个开源、低成本的自动化手抓（AutoRG）拉平台，减少了实验者的干预和可变性。我们使用AutoRG评估了大鼠在七个逐步困难阶段的手握功能。我们利用直接早期基因编码的Arc（活性调节细胞骨架相关蛋白）的体积成像绘制了大鼠大脑中AutoRG和srt激活的运动回路。大鼠在AutoRG训练后表现出强健的前肢伸展和牵拉行为。实时记录单个到达事件的可靠力与时间响应，用于导出几个次要功能性能度量。此外，我们首次证明，在30分钟的训练时间内，AutoRG和SRT在前肢尾侧区（CFA）、前肢吻侧区（RFA）和感觉运动区（S1）都有相似的神经反应。AutoRG是第一个低成本、开源的牵引系统，设计用于扩大意志前肢运动功能测试和表征啮齿动物的伸手行为。在SRT和AutoRG评估后，在大鼠运动皮层中观察到的神经元激活模式的相似性验证了AutoRG是传统SRT和昂贵的商业系统的严格表征，可扩展的替代方案。
Rodent reach-to-grasp function assessment is a translationally powerful model for evaluating neurological function impairments and recovery responses. Existing assessment platforms are experimenter-dependent, costly, or low-throughput with limited output measures. Further, a direct histologic comparison of neural activation has never been conducted between any novel, automated platform and the well-established single pellet skilled reach task (SRT). To address these technological and knowledge gaps, we designed an open-source, low-cost Automatized Reach-to-Grasp (AutoRG) pull platform that reduces experimenter interventions and variability. We assessed reach-to-grasp function in rats across seven progressively difficult stages using AutoRG. We mapped AutoRG and SRT-activated motor circuitries in the rat brain using volumetric imaging of the immediate early gene-encoded Arc (activity-regulated cytoskeleton-associated) protein. Rats demonstrated robust forelimb reaching and pulling behavior after training in AutoRG. Reliable force versus time responses were recorded for individual reach events in real time, which were used to derive several secondary functional measures of performance. Moreover, we provide the first demonstration that for a training period of 30 min, AutoRG and SRT both engage similar neural responses in the caudal forelimb area (CFA), rostral forelimb area (RFA), and sensorimotor area (S1). AutoRG is the first low-cost, open-source pull system designed for the scale-up of volitional forelimb motor function testing and characterization of rodent reaching behavior. The similarities in neuronal activation patterns observed in the rat motor cortex after SRT and AutoRG assessments validate the AutoRG as a rigorously characterized, scalable alternative to the conventional SRT and expensive commercial systems.
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影响因子：
4.2
作者：
Hays SA;Ruiz A;Bethea T;Khodaparast N;Carmel JB;Rennaker RL 2nd;Kilgard MP
通讯作者：
Kilgard MP
影响因子：
5.3
作者：
Bosch-Bouju, Clementine;Smither, Roseanna A.;Parr-Brownlie, Louise C.
通讯作者：
Parr-Brownlie, Louise C.
影响因子：
17.1
作者：
Goodman, Steven N.;Fanelli, Daniele;Ioannidis, John P. A.
通讯作者：
Ioannidis, John P. A.
影响因子：
6.1
作者：
Khodaparast, N.;Hays, S. A.;Kilgard, M. P.
通讯作者：
Kilgard, M. P.
影响因子：
3.4
作者：
Bova, Alexandra;Ferris, Kenneth;Leventhal, Daniel K.
通讯作者：
Leventhal, Daniel K.