Comparative analysis of swine leukocyte antigen gene diversity in European farmed pigs.
Comparative analysis of swine leukocyte antigen gene diversity in European farmed pigs.
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DOI：
10.1111/age.13090
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发表时间：
2021-08
期刊：
影响因子：
2.4
通讯作者：
Saalmueller A
中科院分区：
文献类型：
--
作者：
Hammer SE;Duckova T;Groiss S;Stadler M;Jensen-Waern M;Golde WT;Gimsa U;Saalmueller A
在欧洲，猪是重要的经济家畜，并已成为生物医学研究、移植和再生医学研究的首选临床前大型动物模型。随着猪作为人类疾病模型和器官移植实验的广泛使用，以及它们在感染研究和兽医疫苗设计中的使用，对猪白细胞抗原(SLA)分型的需求日益增加。在本研究中，我们用低分辨率(LR)SLA单倍型技术对代表9个商品猪品系的549头养猪的SLA I类(SLA-1、SLA-2、SLA-3)和II类(DRB1、DQB1、DQA)基因进行了特征分析。在研究的队列中，总共鉴定出50个I类单倍型和37个II类单倍型。最常见的SLA I类单倍型LR-04.0(SLA-1*04XX-SLA-3*04XX(04：04)-SLA-2*04XX)和LR-32.0(SLA-1*07XX-SLA-3*04XX(04：04)-SLA-2*02XX)的出现频率分别为11.02%和8.20%。在SLAⅡ类中，最常见的单倍型LR-0.15b(DRB1*04XX(04:05/04:06)‐DQB1*02XX(02:02)‐DQA*02XX)和LR-0.12(DRB1*06XX-DQB1*07XX-DQA*01XX)的出现频率分别为14.37%和12.46%。同时，我们的实验室参与了几项疫苗相关性研究(例如猪繁殖与呼吸综合征病毒、猪瘟病毒、口蹄疫病毒和甲型猪流感病毒)，阐明了T细胞应答中的免疫优势，其抗原特异性取决于某些SLA-I和SLA-II单倍型。此外，这些SLA免疫反应相关性有助于定制疫苗的开发，因为SLA-I LR-04.0和LR-32.0以及SLA-II LR-0.15b和LR-0.12是欧洲饲养猪中高度丰富的单倍型。
In Europe, swine represent economically important farm animals and furthermore have become a preferred preclinical large animal model for biomedical studies, transplantation and regenerative medicine research. The need for typing of the swine leukocyte antigen (SLA) is increasing with the expanded use of pigs as models for human diseases and organ‐transplantation experiments and their use in infection studies and for design of veterinary vaccines. In this study, we characterised the SLA class I (SLA‐1, SLA‐2, SLA‐3) and class II (DRB1, DQB1, DQA) genes of 549 farmed pigs representing nine commercial pig lines by low‐resolution (Lr) SLA haplotyping. In total, 50 class I and 37 class II haplotypes were identified in the studied cohort. The most common SLA class I haplotypes Lr‐04.0 (SLA‐1*04XX‐SLA‐3*04XX(04:04)‐SLA‐2*04XX) and Lr‐32.0 (SLA‐1*07XX‐SLA‐3*04XX(04:04)‐SLA‐2*02XX) occurred at frequencies of 11.02 and 8.20% respectively. For SLA class II, the most prevalent haplotypes Lr‐0.15b (DRB1*04XX(04:05/04:06)‐DQB1*02XX(02:02)‐DQA*02XX) and Lr‐0.12 (DRB1*06XX‐DQB1*07XX‐DQA*01XX) occurred at frequencies of 14.37 and 12.46% respectively. Meanwhile, our laboratory has contributed to several vaccine correlation studies (e.g. Porcine Reproductive and Respiratory Syndrome Virus, Classical Swine Fever Virus, Foot‐and‐Mouth Disease Virus and Swine Influenza A Virus) elucidating the immunodominance in the T‐cell response with antigen specificity dependent on certain SLA‐I and SLA‐II haplotypes. Moreover, these SLA–immune response correlations could facilitate tailored vaccine development, as SLA‐I Lr‐04.0 and Lr‐32.0 as well as SLA‐II Lr‐0.15b and Lr‐0.12 are highly abundant haplotypes in European farmed pigs.
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影响因子：
5.4
作者：
Talker SC;Stadler M;Koinig HC;Mair KH;Rodríguez-Gómez IM;Graage R;Zell R;Dürrwald R;Starick E;Harder T;Weissenböck H;Lamp B;Hammer SE;Ladinig A;Saalmüller A;Gerner W
通讯作者：
Gerner W
影响因子：
7.8
作者：
de León P;Cañas-Arranz R;Saez Y;Forner M;Defaus S;Cuadra D;Bustos MJ;Torres E;Andreu D;Blanco E;Sobrino F;Hammer SE
通讯作者：
Hammer SE
影响因子：
3.7
作者：
Franzoni G;Kurkure NV;Essler SE;Pedrera M;Everett HE;Bodman-Smith KB;Crooke HR;Graham SP
通讯作者：
Graham SP
DOI：
10.1146/annurev-animal-020518-115014
发表时间：
2020-01-01
期刊：
ANNUAL REVIEW OF ANIMAL BIOSCIENCES, VOL 8, 2020
影响因子：
--
作者：
Hammer, Sabine E.;Ho, Chak-Sum;Lunney, Joan K.
通讯作者：
Lunney, Joan K.
感染了猪流感A的猪中多功能CD4+和CD8β+ T细胞的大小和动力学。
影响因子：
4.4
作者：
Talker SC;Koinig HC;Stadler M;Graage R;Klingler E;Ladinig A;Mair KH;Hammer SE;Weissenböck H;Dürrwald R;Ritzmann M;Saalmüller A;Gerner W
通讯作者：
Gerner W