Magnitude and kinetics of multifunctional CD4+ and CD8β+ T cells in pigs infected with swine influenza A virus.
Magnitude and kinetics of multifunctional CD4+ and CD8β+ T cells in pigs infected with swine influenza A virus.
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感染了猪流感A的猪中多功能CD4+和CD8β+ T细胞的大小和动力学。
DOI：
10.1186/s13567-015-0182-3
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发表时间：
2015-05-14
影响因子：
4.4
通讯作者：
Gerner W
中科院分区：
文献类型：
--
作者：
Talker SC;Koinig HC;Stadler M;Graage R;Klingler E;Ladinig A;Mair KH;Hammer SE;Weissenböck H;Dürrwald R;Ritzmann M;Saalmüller A;Gerner W
虽然猪是甲型流感病毒的天然宿主，但迄今为止，猪T细胞对猪甲型流感病毒（FLUAVsw）感染的反应特征很差。我们研究了从H1 N2 FLUAVsw分离株经肠道感染的仔猪中分离的CD 4+和CD 8 β+ T细胞中Ki-67表达和FLUAVsw特异性IFN-γ、TNF-α和IL-2的产生。在初次感染后1或2周，所有感染动物的血液中均存在IFN-γ+TNF-α+IL-2+多功能CD 4 + T细胞，在同源二次感染后，6只动物中有4只动物的频率增加。这些细胞比仅产生单一细胞因子的CD 4 + T细胞产生更高量的IFN-γ、TNF-α和IL-2。绝大多数产生精氨酸的CD 4 + T细胞表达CD 8 α，这是一种与猪CD 4 + T细胞活化和记忆形成相关的标志物。CD 27表达的分析表明，FLUAVsw特异性CD 4 + T细胞包括中枢记忆和效应记忆群体。感染后1周，6只动物中有3只显示血液中Ki-67+穿孔素+CD 8 β+ T细胞的强烈增加。血液来源的FLUAVsw特异性CD 8 β+ T细胞可以在体外扩增阶段后鉴定，并且在CD 107 a表达和IFN-γ和TNF-α的共产生方面是多功能的。这些数据表明，多功能T细胞响应于猪的FLUAVsw感染而产生，支持T细胞有助于有效控制感染的观点。本文的在线版本（doi：10.1186/s13567-015-0182-3）包含补充材料，可供授权用户使用。
Although swine are natural hosts for influenza A viruses, the porcine T-cell response to swine influenza A virus (FLUAVsw) infection has been poorly characterized so far. We have studied Ki-67 expression and FLUAVsw-specific production of IFN-γ, TNF-α and IL-2 in CD4+ and CD8β+ T cells isolated from piglets that had been intratracheally infected with a H1N2 FLUAVsw isolate. IFN-γ+TNF-α+IL-2+ multifunctional CD4+ T cells were present in the blood of all infected animals at one or two weeks after primary infection and their frequency increased in four out of six animals after homologous secondary infection. These cells produced higher amounts of IFN-γ, TNF-α and IL-2 than did CD4+ T cells that only produced a single cytokine. The vast majority of cytokine-producing CD4+ T cells expressed CD8α, a marker associated with activation and memory formation in porcine CD4+ T cells. Analysis of CD27 expression suggested that FLUAVsw-specific CD4+ T cells included both central memory and effector memory populations. Three out of six animals showed a strong increase of Ki-67+perforin+ CD8β+ T cells in blood one week post infection. Blood-derived FLUAVsw-specific CD8β+ T cells could be identified after an in vitro expansion phase and were multifunctional in terms of CD107a expression and co-production of IFN-γ and TNF-α. These data show that multifunctional T cells are generated in response to FLUAVsw infection of pigs, supporting the idea that T cells contribute to the efficient control of infection. The online version of this article (doi:10.1186/s13567-015-0182-3) contains supplementary material, which is available to authorized users.
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影响因子：
2.7
作者：
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影响因子：
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作者：
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通讯作者：
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