Proteomics-based analysis of invasion-related proteins in malignant gliomas.
Proteomics-based analysis of invasion-related proteins in malignant gliomas.
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DOI：
10.1111/j.1440-1789.2012.01361.x
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发表时间：
2013-06
期刊：
影响因子：
--
通讯作者：
Date I
中科院分区：
文献类型：
--
作者：
Maruo T;Ichikawa T;Kanzaki H;Inoue S;Kurozumi K;Onishi M;Yoshida K;Kambara H;Ouchida M;Shimizu K;Tamaru S;Chiocca EA;Date I
胶质瘤潜在的生物学特征之一是可能广泛侵犯正常脑组织，然而决定这种局部侵袭行为的分子机制仍然知之甚少。为了研究恶性胶质瘤侵袭的分子基础，对两个在大鼠脑内表现不同侵袭表型但具有相似遗传背景的犬脑胶质瘤亚克隆J3T-1和J3T-2进行了蛋白质组学分析。对J3T-1(血管生成依赖性侵袭表型)和J3T-2(血管生成非依赖性侵袭性表型)的全细胞裂解产物进行双向蛋白质电泳。当显著变化被定义为J3T-1和J3T-2之间的光斑强度变化超过1.5倍时，识别出22个明显的斑点。液-质联用分析鉴定了4个在J3T-1中高表达的蛋白质和14个在J3T-2中高表达的蛋白质。鉴定的蛋白质之一是膜联蛋白A2，在J3T-1中的表达水平高于在J3T-2中的表达水平。培养细胞的定量逆转录-聚合酶链式反应和免疫组织化学染色证实了膜联蛋白A2在J3T-1中的高表达。此外，对人胶质母细胞瘤标本的免疫组织化学分析表明，在扩张的血管周围形成簇的肿瘤细胞中，Annexin A2高水平表达。这些结果揭示了这两种细胞系之间蛋白质组特征的差异，这可能与它们不同的侵袭特征有关。因此，膜联蛋白A2可能与血管生成依赖性侵袭有关。
One of the insidious biological features of gliomas is potential to invade normal brain tissue extensively, yet molecular mechanisms that dictate this locally invasive behavior remain poorly understood. To investigate the molecular basis of invasion by malignant gliomas, proteomic analysis was performed using a pair of canine glioma subclones—J3T-1 and J3T-2—that show different invasion phenotypes in rat brains but have similar genetic backgrounds. Two-dimensional protein electrophoresis of whole-cell lysates of J3T-1 (angiogenesis-dependent invasion phenotype) and J3T-2 (angiogenesis-independent invasion phenotype) was performed. Twenty-two distinct spots were recognized when significant alteration was defined as more than 1.5-fold change in spot intensity between J3T-1 and J3T-2. Four proteins that demonstrated increased expression in J3T-1, and 14 proteins that demonstrated increased expression in J3T-2 were identified using liquid chromatography-mass spectrometry analysis. One of the proteins identified was annexin A2, which was expressed at higher levels in J3T-1 than in J3T-2. The higher expression of annexin A2 in J3T-1 was corroborated by quantitative reverse transcription-PCR of the cultured cells and immunohistochemical staining of the rat brain tumors. Moreover, immunohistochemical analysis of human glioblastoma specimens showed that annexin A2 was expressed at high levels in the tumor cells that formed clusters around dilated vessels. These results reveal differences in the proteomic profiles between these two cell lines that might correlate with their different invasion profiles. Thus, annexin A2 may be related to angiogenesis-dependent invasion.
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影响因子：
6.4
作者：
Ichikawa, T;Tamiya, T;Ohmoto, T
通讯作者：
Ohmoto, T
影响因子：
8.8
作者：
通讯作者：
--
影响因子：
8.8
作者：
Duncan, R.;Carpenter, B.;Main, L. C.;Telfer, C.;Murray, G. I.
通讯作者：
Murray, G. I.
影响因子：
11.2
作者：
Iwadate, Y;Sakaida, T;Yamaura, A
通讯作者：
Yamaura, A
DOI：
10.1016/j.bbrc.2004.07.124
发表时间：
2004-10-01
影响因子：
3.1
作者：
Hayes, MJ;Moss, SE
通讯作者：
Moss, SE