Polyphenols extracted from huangjiu have anti-inflammatory activity in lipopolysaccharide stimulated RAW264.7 cells.
Polyphenols extracted from huangjiu have anti-inflammatory activity in lipopolysaccharide stimulated RAW264.7 cells.
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从黄酒中提取的多酚对脂多糖刺激的RAW264.7细胞具有抗炎活性
DOI：
10.1039/c8ra09671f
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发表时间：
2019-02-11
期刊：
影响因子：
3.9
通讯作者：
Mao, Jian
中科院分区：
文献类型：
--
作者：
Peng, Lin;Ai-lati, Aisikaer;Ji, Zhongwei;Chen, Shuguang;Mao, Jian
采用乙酸乙酯+乙醇提取结合HPD 400树脂纯化的方法提取黄酒多酚。经提取纯化后，多酚得率为22.57%，蛋白质去除率为90.57%，糖去除率为97.99%。HPLC分析表明，（+）-儿茶素（91.33 μg mL−1）是11种检测到的多酚中的主要酚类化合物。在LPS刺激的RAW 264.7细胞中，HPE通过抑制NO和促炎细胞因子（TNF-α、白细胞介素IL-6和IL-1β）的产生而表现出抗炎作用。HPE的抗炎作用与抑制iNOS表达、抑制NF-κB易位至细胞核以及抑制IκB和MAPK家族蛋白（例如p-38、Erk 1/2和JNK）的磷酸化有关。此外，HPE的抗炎作用还与Nrf 2和HO-1的激活有关。含有主要多酚（+）-儿茶素的HPE通过NF-κB途径和MAPK信号传导表现出抗炎活性。
In the present study, an extraction method, combining extraction by ethyl acetate + ethanol and purification by HPD400 resin, was established to obtain huangjiu polyphenol extract (HPE). After extraction and purification, the polyphenol yield was 22.57%, and 90.57% protein and 97.99% sugar were removed. HPLC analysis indicated that (+)-catechin (91.33 μg mL−1) was the predominant phenolic compound among the 11 detected polyphenols. In LPS-stimulated RAW264.7 cells, HPE exhibits anti-inflammatory effects by inhibiting the production of NO and pro-inflammatory cytokines (TNF-α, interleukin IL-6 and IL-1β). The anti-inflammatory effect of HPE is associated with the inhibition of iNOS expression, the suppression of NF-κB translocation to the nucleus, and the inhibition of the phosphorylation of IκB and the MAPK family proteins, e.g. p-38, Erk 1/2, and JNK. Moreover, the activation of Nrf2 and HO-1 is also related to the anti-inflammatory effect of HPE. HPE with the predominant polyphenol of (+)-catechin exhibits anti-inflammatory activity through the NF-κB pathway and MAPK signaling.
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影响因子：
6.1
作者：
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影响因子：
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影响因子：
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作者：
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影响因子：
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作者：
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