Bioactive Scaffolds for Regeneration of Cartilage and Subchondral Bone Interface.
Bioactive Scaffolds for Regeneration of Cartilage and Subchondral Bone Interface.
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作者：
Deng C;Zhu H;Li J;Feng C;Yao Q;Wang L;Chang J;Wu C
骨关节炎（OA）引起的软骨损伤常延伸至软骨下骨。这对软骨和软骨下骨两种组织的同时再生具有重要意义。3D打印Sr5（PO4）2SiO4（SPS）生物活性陶瓷支架可以实现软骨和软骨下骨再生的目的。我们假设，锶（Sr）和硅（Si）离子释放的SPS支架在骨软骨缺损重建中起着至关重要的作用。方法：采用3D打印技术制备SPS生物活性陶瓷支架。利用扫描电镜和等离子体发射光谱仪研究了SPS支架的物理化学性能。观察SPS生物活性陶瓷对体外培养的兔软骨细胞增殖和成熟的影响。在体内研究SPS支架对软骨和软骨下骨再生的刺激作用。结果如下：SPS支架显著刺激软骨细胞增殖，SPS提取物明显促进软骨细胞的成熟，并从OA保存软骨细胞。SPS支架能明显促进骨软骨缺损的再生。软骨与软骨下骨之间复杂的界面显微结构得到明显重建。其机制可能与Sr、Si离子通过激活HIF通路促进软骨再生，通过激活Wnt通路促进软骨下骨重建，以及通过诱导自噬和抑制hedgehog通路保护软骨细胞免受OA损伤有关。结论：我们的研究结果表明，SPS支架可以帮助骨软骨缺损重建，并很好地重建软骨和软骨下骨之间的复杂界面，这是一个有前途的策略，骨软骨缺损再生。
The cartilage lesion resulting from osteoarthritis (OA) always extends into subchondral bone. It is of great importance for simultaneous regeneration of two tissues of cartilage and subchondral bone. 3D-printed Sr5(PO4)2SiO4 (SPS) bioactive ceramic scaffolds may achieve the aim of regenerating both of cartilage and subchondral bone. We hypothesized that strontium (Sr) and silicon (Si) ions released from SPS scaffolds play a crucial role in osteochondral defect reconstruction. Methods: SPS bioactive ceramic scaffolds were fabricated by a 3D-printing method. The SEM and ICPAES were used to investigate the physicochemical properties of SPS scaffolds. The proliferation and maturation of rabbit chondrocytes stimulated by SPS bioactive ceramics were measured in vitro. The stimulatory effect of SPS scaffolds for cartilage and subchondral bone regeneration was investigated in vivo. Results: SPS scaffolds significantly stimulated chondrocyte proliferation, and SPS extracts distinctly enhanced the maturation of chondrocytes and preserved chondrocytes from OA. SPS scaffolds markedly promoted the regeneration of osteochondral defects. The complex interface microstructure between cartilage and subchondral bone was obviously reconstructed. The underlying mechanism may be related to Sr and Si ions stimulating cartilage regeneration by activating HIF pathway and promoting subchondral bone reconstruction through activating Wnt pathway, as well as preserving chondrocytes from OA via inducing autophagy and inhibiting hedgehog pathway. Conclusion: Our findings suggest that SPS scaffolds can help osteochondral defect reconstruction and well reconstruct the complex interface between cartilage and subchondral bone, which represents a promising strategy for osteochondral defect regeneration.
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影响因子：
2.8
作者：
Castro NJ;Patel R;Zhang LG
通讯作者：
Zhang LG
影响因子：
4.6
作者：
Fleury A;Hoch L;Martinez MC;Faure H;Taddei M;Petricci E;Manetti F;Girard N;Mann A;Jacques C;Larghero J;Ruat M;Andriantsitohaina R;Le Lay S
通讯作者：
Le Lay S
锶取代硅酸钙生物活性陶瓷增强骨质疏松骨再生
影响因子：
14
作者：
Lin, Kaili;Xia, Lunguo;Chang, Jiang
通讯作者：
Chang, Jiang
影响因子：
2.6
作者：
Ferguson, C;Alpern, E;Helms, JA
通讯作者：
Helms, JA
影响因子：
3.9
作者：
Calomme, MR;VandenBerghe, DA
通讯作者：
VandenBerghe, DA