Preliminary comparison of grating-based and in-line phase contrast X-ray imaging with synchrotron radiation for mouse kidney at TOMCAT
Preliminary comparison of grating-based and in-line phase contrast X-ray imaging with synchrotron radiation for mouse kidney at TOMCAT
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TOMCAT 小鼠肾脏光栅相衬 X 射线成像与同步辐射的初步比较
DOI：
10.1088/1748-0221/8/06/c06003
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发表时间：
2013-06
影响因子：
1.3
通讯作者：
Xu, L.X.
中科院分区：
文献类型：
--
作者：
Sun, J.;Liu, P.;Irvine, S.;Pinzer, B.;Stampanoni, M.;Xu, L.X.
在没有造影剂的情况下，相位对比度成像在揭示生物标本内部的详细结构方面具有优势。基于光栅的差动相位对比度(DPC)和同轴相位对比度(ILPC)X射线成像是瑞士光源(SLS)的层析显微镜和相干放射学实验(TOMCAT)光束线上经常使用的两种方式。本文初步比较了两种不同类型的相衬成像对小鼠肾脏微结构的分辨能力。三维重建显示，两种成像方式均能清晰显示肾脏的微血管、肾小管等微结构。这两种技术可以被视为互补的。对于密度变化非常小的较大特征，DPC是理想的方法。在剂量和时间限制可能禁止DPC所需的多个步骤的情况下，当重点放在更精细的特征上时，ILPC方法可能被认为是更可行的替代方法。此外，高分辨率ILPC图像与组织学结果具有可比性。
Phase contrast imaging has been demonstrated to be advantageous in revealing detailed structures inside biological specimens without contrast agents. Grating-based differential phase contrast (DPC) and in-line phase contrast (ILPC) X-ray imaging are the two modalities frequently used at the beamline of TOmographic Microscopy and Coherent rAdiology experimenTs (TOMCAT) at the Swiss Light Source (SLS). In this paper, we preliminarily compared the abilities of two types of phase contrast imaging in distinguishing micro structures in mouse kidneys. The 3D reconstructions showed that the microstructures in kidney, such as micro vessels and renal tubules, were displayed clearly with both imaging modalities. The two techniques may be viewed as complementary. For larger features with very small density variations DPC is the desirable method. In cases where dose and time limits may prohibit the multiple steps required for DPC, and when the focus is on finer features, the ILPC method may be considered as a more viable alternative. Moreover, high resolution ILPC images are comparable with histological results.
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影响因子：
3.5
作者：
Hwu, Y;Tsai, WL;Seong, JK
通讯作者：
Seong, JK
影响因子：
5
作者：
W. Welford
通讯作者：
W. Welford
影响因子：
19.6
作者：
Pfeiffer, F;Weitkamp, T;David, C
通讯作者：
David, C
影响因子：
4
作者：
Zanette, I.;Bech, M.;Weitkamp, T.
通讯作者：
Weitkamp, T.
影响因子：
2
作者：
Engelhardt, M.;Kottler, C.;Pfeiffer, F.
通讯作者：
Pfeiffer, F.