The Canonical E2Fs Are Required for Germline Development in Arabidopsis.
The Canonical E2Fs Are Required for Germline Development in Arabidopsis.
复制标题
拟南芥种系发育需要规范 E2F
DOI：
10.3389/fpls.2018.00638
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发表时间：
2018
影响因子：
5.6
通讯作者：
Wang S
中科院分区：
文献类型：
--
作者：
Yao X;Yang H;Zhu Y;Xue J;Wang T;Song T;Yang Z;Wang S
许多细胞命运的决定，包括细胞分裂、细胞分化和细胞程序性死亡，强烈地发生在植物种系的发育过程中。这些细胞命运的决定是如何受到监管的，在很大程度上仍不清楚。转录因子E2F是核心细胞周期调节因子。在这里，我们证明了拟南芥中典型的E2F，包括E2Fa、E2Fb和E2Fc，在植物种系发育中扮演着多余的角色。E2fa e2fb e2fc(E2Fabc)三重突变体是不育的，尽管其营养发育看起来是正常的。一方面，e2Fabc小孢子在花粉有丝分裂过程中发生细胞死亡。小孢子在双细胞期开始死亡。到三细胞期，大部分e2Fabc小孢子退化。另一方面，野生型胚珠通常有一个大孢子母细胞，而大多数e2Fabc胚珠有两到三个大孢子母细胞。随后e2Fabc突变体的雌配子发生被中止，胚囊中的液泡严重受损。传递效率分析表明，雄配子体和雌配子体的典型E2F对植物配子发生是必不可少的。我们的研究表明，典型的E2F是植物种系发育所必需的，特别是花粉有丝分裂和孢原细胞(AC)-MMC的转变。
A number of cell fate determinations, including cell division, cell differentiation, and programmed cell death, intensely occur during plant germline development. How these cell fate determinations are regulated remains largely unclear. The transcription factor E2F is a core cell cycle regulator. Here we show that the Arabidopsis canonical E2Fs, including E2Fa, E2Fb, and E2Fc, play a redundant role in plant germline development. The e2fa e2fb e2fc (e2fabc) triple mutant is sterile, although its vegetative development appears normal. On the one hand, the e2fabc microspores undergo cell death during pollen mitosis. Microspores start to die at the bicellular stage. By the tricellular stage, the majority of the e2fabc microspores are degenerated. On the other hand, a wild type ovule often has one megaspore mother cell (MMC), whereas the majority of e2fabc ovules have two to three MMCs. The subsequent female gametogenesis of e2fabc mutant is aborted and the vacuole is severely impaired in the embryo sac. Analysis of transmission efficiency showed that the canonical E2Fs from both male and female gametophyte are essential for plant gametogenesis. Our study reveals that the canonical E2Fs are required for plant germline development, especially the pollen mitosis and the archesporial cell (AC)-MMC transition.
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影响因子：
11.6
作者：
Liu, Yiyang;Lai, Jianbin;Yang, Chengwei
通讯作者：
Yang, Chengwei
影响因子：
7.2
作者：
Cheng, Yan;Cao, Ling;Zhou, Yongming
通讯作者：
Zhou, Yongming
影响因子：
11.6
作者：
del Pozo, JC;Boniotti, MB;Gutierrez, C
通讯作者：
Gutierrez, C
影响因子：
9.2
作者：
Lieber, Diana;Lora, Jorge;Laux, Thomas
通讯作者：
Laux, Thomas
DOI：
10.1073/pnas.0810992106
发表时间：
2009-04-28
影响因子：
11.1
作者：
Chen, Zhong;Hafidh, Said;Berger, Frederic
通讯作者：
Berger, Frederic