Genetic diversity of the enterohaemolysin gene (ehxA) in non-O157 Shiga toxin-producing Escherichia coli strains in China.
Genetic diversity of the enterohaemolysin gene (ehxA) in non-O157 Shiga toxin-producing Escherichia coli strains in China.
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中国非O157产志贺毒素大肠杆菌菌株肠溶血素基因（ehxA）的遗传多样性
DOI：
10.1038/s41598-018-22699-7
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发表时间：
2018-03-09
影响因子：
4.6
通讯作者：
Xiong Y
中科院分区：
文献类型：
--
作者：
Fu S;Bai X;Fan R;Sun H;Xu Y;Xiong Y
非O 157型滋贺毒素大肠埃希菌（STEC）是一种重要的食源性肠道致病菌。该疾病的标志是产生滋贺毒素;然而，还有其他毒力因子有助于STEC的发病机制。本研究旨在调查肠溶血素基因ehxA在来自人类、动物和食物来源的非O 157 STEC菌株中的患病率和遗传多样性。在434株非O 157 STEC中，有138株（31.8%）扩增出ehxA基因，其中36株（31.8%）有ehxA序列。基于ehxA序列分析，三个系统发育ehxA组（I，II和III）的确定。ehxA组和来源，血清型和毒力基因谱之间的相关性进行了观察。EhxA组II菌株大多为脑膜炎患者来源，与EhxA组I和组III菌株相比，可能表现出更高的致病潜力。在138株EhxA阳性菌株中鉴定出I1-IG、FI B、K、F和B/O 5种类型的复制子，其中toxB、katP和espP基因的携带率分别为3.6%、5.8%和52.2%，表明含EhxA质粒在非O 157 STEC菌株中存在明显的遗传多样性。基于序列的ehxA基因分型可能是重要的现代菌株分型和非O 157 STEC感染的流行病学监测。
Non-O157 Shiga toxin-producing Escherichia coli (STEC) is increasingly recognized as an important enteric foodborne pathogen. The hallmark of the disease is the production of Shiga toxins; however, there are other virulence factors that contribute to the pathogenesis of STEC. This study aimed to investigate the prevalence and genetic diversity of the enterohaemolysin gene, ehxA, among non-O157 STEC strains from human, animal, and food sources. The ehxA gene was amplified from 138 (31.8%) of 434 non-O157 STEC strains, among which 36 unique ehxA sequences were identified. Based on ehxA sequence analysis, three phylogenetic ehxA groups (I II, and III) were determined. Correlations between ehxA groups and sources, serotypes, and virulent gene profiles were observed. The ehxA group II strains were mostly diarrhoeal patient-derived and may demonstrate higher pathogenic potential compared with the ehxA group I and group III strains. Five types of replicons (I1-Ig, FIB, K, F, and B/O) were identified in the 138 ehxA-positive strains, and 3.6%, 5.8%, and 52.2% of the strains harboured toxB, katP and espP genes, respectively, implying marked genetic diversity of ehxA containing plasmids in non-O157 STEC strains. Sequence-based ehxA genotyping might be important in modern strain typing and in epidemiological surveillance of non-O157 STEC infections.
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DOI：
10.1016/j.ijfoodmicro.2015.01.018
发表时间：
2015-05-04
影响因子：
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作者：
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发表时间：
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期刊：
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影响因子：
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作者：
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影响因子：
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