Oxygen-dependent laser inactivation of murine norovirus using visible light lasers.
Oxygen-dependent laser inactivation of murine norovirus using visible light lasers.
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使用可见光激光器，氧气依赖性激光灭活了鼠病毒。
DOI：
10.1186/s12985-018-1019-2
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发表时间：
2018-07-31
期刊：
影响因子：
4.8
通讯作者：
Johnson A
中科院分区：
文献类型：
--
作者：
Kingsley D;Kuis R;Perez R;Basaldua I;Burkins P;Marcano A;Johnson A
先前的研究表明，超短脉冲（USP） 425 nm激光能够灭活小鼠诺如病毒（MNV: Virol）。J. 11:20)，可能是通过脉冲受激拉曼散射（ISRS）机制，并且不会实质上损害人类血浆蛋白（PLOS One 9:11）。在这里，进一步研究病毒灭活激光进行。在这项研究中，我们评估了MNV的失活是否只发生在USP波长425 nm，或者是否发生在其他可见波长，使用频率翻倍的可调谐锁模Ti-Sapphire激光器产生400、408、425、450、465和510 nm波长的飞秒脉冲。连续波（CW）激光器也被应用。单线态氧增强剂用于评估MNV对单线态氧的敏感性，氧猝灭剂用于评估与未处理对照相比对病毒灭活的影响。在408、425或450 nm飞秒脉冲光的平均功率为150 mW照射3小时后，MNV pfu发生失活。因此，结果表明激光灭活病毒的机制不是波长特异性的。此外，我们还表明，使用功率相似的连续波（CW）激光在408 nm处照射也会产生大量的MNV失活，这表明失活不需要USP。使用光敏剂、核黄素、玫瑰红和亚甲基蓝产生单线态氧，大大提高了失活的效率。结果表明，相对低功率的蓝色激光作用产生单线态氧是激光诱导失活的光化学机制。结果表明，短寿命活性氧（如单线态氧）的形成是由可见激光引起的，而不是通过诱导共振振动的ISRS机制引起的。
Previous work indicated that an ultrashort pulse (USP) 425 nm laser is capable of inactivating murine norovirus (MNV: Virol. J. 11:20), perhaps via an impulsive stimulated Raman scattering (ISRS) mechanism, and does not substantially damage human plasma proteins (PLOS One 9:11). Here, further investigation of virus inactivation by laser light is performed. In this study, we evaluate whether inactivation of MNV is specific to the USP wavelength of 425 nm, or if it occurs at other visible wavelengths, using a tunable mode-locked Ti-Sapphire laser that has been frequency doubled to generate femtosecond pulses at wavelengths of 400, 408, 425, 450, 465, and 510 nm. Continuous Wave (CW) lasers are also applied. Singlet oxygen enhancers are used to evaluate the sensitivity of MNV to singlet oxygen and oxygen quenchers are used to evaluate effects on virus inactivation as compared to untreated controls. > 3 log10 inactivation of MNV pfu occurs after irradiation with an average power of 150 mW at wavelengths of 408, 425 or 450 nm femtosecond-pulsed light for 3 h. Thus results suggest that the mechanism by which a laser inactivates the virus is not wavelength-specific. Furthermore, we also show that irradiation using a continuous wave (CW) laser of similar power at 408 nm also yields substantial MNV inactivation indicating that inactivation does not require a USP. Use of photosensitizers, riboflavin, rose bengal and methylene blue that generate singlet oxygen substantially improves the efficiency of the inactivation. The results indicate a photochemical mechanism of the laser-induced inactivation where the action of relatively low power blue laser light generates singlet oxygen. Results suggest formation of short-lived reactive oxygen species such as singlet oxygen by visible laser light as the cause of virus inactivation rather than via an ISRS mechanism which induces resonant vibrations.
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影响因子：
2.4
作者：
Kim, S. Y.;Ko, G.
通讯作者：
Ko, G.
影响因子：
11.8
作者：
Hall AJ;Lopman BA;Payne DC;Patel MM;Gastañaduy PA;Vinjé J;Parashar UD
通讯作者：
Parashar UD
DOI：
10.1615/jenvironpatholtoxicoloncol.v25.i1-2.320
发表时间：
2006-01-01
影响因子：
2.4
作者：
Jori, G
通讯作者：
Jori, G
影响因子：
12.8
作者：
Kohn, Tamar;Mattle, Michael J.;Vione, Davide
通讯作者：
Vione, Davide
影响因子：
3.9
作者：
Huang, R;Choe, E;Min, DB
通讯作者：
Min, DB