Role of ischemic preconditioning on ischemia-reperfusion injury of the lung.
Role of ischemic preconditioning on ischemia-reperfusion injury of the lung.
复制标题
缺血预处理对肺缺血再灌注损伤的作用。
DOI：
10.1378/chest.115.6.1672
复制
发表时间：
1999
期刊：
影响因子：
9.6
通讯作者：
S. Kalaycioğlu
中科院分区：
文献类型：
--
作者：
H. Soncul;E. Öz;S. Kalaycioğlu
学习目标
肺缺血再灌注损伤常发生在体外循环术后、肺血栓栓塞切除术后，尤其是肺移植过程中。预处理对心脏、肝脏、骨骼和各种其他器官的保护作用之前已经被评估过。在这项比较研究中，我们使用离体豚鼠肺来显示预处理对肺缺血的影响。
方法
将肺（每组 n = 10）安装在改良的 Langendorff 灌注装置上，并用 Krebs-Henseleit 溶液灌注 30 分钟。实验组采用缺血预处理（5分钟缺血+5分钟灌注，两次）。常温缺血3小时后，肺再灌注30分钟。在缺血期之前和之后以及再灌注结束时测量组织和灌注液的肺动脉压力以及丙二醛（MDA）和谷胱甘肽（GSH）水平。实验结束时，对每组中随机选择的三只动物的肺部进行电子显微镜评估。
结果
再灌注后实验组组织和灌流液的MDA和GSH水平均下降，但GSH水平的下降并未达到统计学显着性。再灌注后预处理组肺动脉压增加较低。
结论
我们的数据表明，肺的缺血预处理可能对缺血再灌注损伤具有保护作用。
STUDY OBJECTIVES
Ischemia-reperfusion injury of the lung frequently occurs after cardiopulmonary bypass, after pulmonary thromboembolectomy, and especially during lung transplantation. The protective effects of preconditioning on the heart, liver, bones, and various other organs have been previously evaluated. In this comparative study, we used isolated guinea pig lungs to show the effects of preconditioning on lung ischemia.
METHODS
The lungs (n = 10 in each group) were mounted on a modified Langendorff perfusion apparatus and perfused by Krebs-Henseleit solution for 30 min. We applied an ischemic preconditioning (5 min ischemia + 5 min perfusion, two times) in the experimental group. After 3 h of normothermic ischemia, the lungs were reperfused for 30 min. Pulmonary artery pressures and malondialdehyde (MDA) and glutathione (GSH) levels of the tissue and the perfusate were measured before and after the ischemic period and also at the end of reperfusion. Electron microscopic evaluation was done on randomly selected lungs of three animals in each group at the end of the experiment.
RESULTS
Both MDA and GSH levels of tissue and perfusate decreased in the experimental group after reperfusion, although the reduction in GSH levels did not reach statistical significance. The increase in pulmonary artery pressure was lower in the preconditioning group after reperfusion.
CONCLUSIONS
Our data showed that ischemic preconditioning of the lung may have a protective effect in ischemic-reperfusion injury.
缺氧和复氧对肺谷胱甘肽系统的影响。
DOI：
10.1152/ajpheart.1990.259.2.h518
发表时间：
1990
期刊：
The American journal of physiology
影响因子：
--
作者：
Jackson,RM;Veal,CF
通讯作者：
Veal,CF
离体大鼠肺的氧依赖性再灌注损伤。
DOI：
10.1152/jappl.1992.72.4.1454
发表时间：
1992
期刊：
Journal of applied physiology (Bethesda, Md. : 1985)
影响因子：
--
作者：
Eckenhoff,RG;Dodia,C;Tan,Z;Fisher,AB
通讯作者：
Fisher,AB