Anti-inflammatory evaluation of the methanolic extract of Taraxacum officinale in LPS-stimulated human umbilical vein endothelial cells.
Anti-inflammatory evaluation of the methanolic extract of Taraxacum officinale in LPS-stimulated human umbilical vein endothelial cells.
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DOI：
10.1186/s12906-017-2022-7
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发表时间：
2017-11-29
影响因子：
--
通讯作者：
Kim HS
中科院分区：
文献类型：
--
作者：
Jeon D;Kim SJ;Kim HS
动脉粥样硬化是一种慢性血管炎性疾病。由于即使是低水平的内毒素血症也是动脉粥样硬化发展的一个强大而独立的危险因素，因此找到针对内毒素血管作用的治疗方法来预防动脉粥样硬化是很重要的。蒲公英（Taraxacum officinale， TO）因其降胆、利尿、抗氧化、抗炎和抗癌特性而被用于医药用途，但其对内皮细胞的抗炎作用尚未确定。我们通过单核细胞粘附和western blot检测，评估了TO过滤甲醇提取物对lps刺激的人脐静脉内皮细胞（HUVECs）的抗炎活性。用100 μg/ml TO预处理HUVECs 1 h，再用1 μg/ml LPS孵育24 h，采用real-time PCR和western blot方法检测靶细胞（促炎因子和粘附分子）mRNA和蛋白表达水平。我们还进行了高效液相色谱分析，以确定甲醇提取物的成分。经TO过滤的甲醇提取物通过降低血管细胞粘附分子-1和单核细胞趋化蛋白-1以及促炎细胞因子的表达，显著抑制lps诱导的内皮细胞-单核细胞相互作用。TO抑制lps诱导的NF-κB核易位，而不影响MAPK的激活。我们的研究结果表明，甲醇提取物可以通过抑制NF-κB通路来减弱lps诱导的内皮细胞活化。这些结果表明了TO在预防血管炎症和动脉粥样硬化方面的潜在临床益处和应用。本文的在线版本（10.1186/s12906-017-2022-7）包含补充内容，仅供授权用户使用。
Atherosclerosis is a chronic vascular inflammatory disease. Since even low-level endotoxemia constitutes a powerful and independent risk factor for the development of atherosclerosis, it is important to find therapies directed against the vascular effects of endotoxin to prevent atherosclerosis. Taraxacum officinale (TO) is used for medicinal purposes because of its choleretic, diuretic, antioxidative, anti-inflammatory, and anti-carcinogenic properties, but its anti-inflammatory effect on endothelial cells has not been established. We evaluated the anti-inflammatory activity of TO filtered methanol extracts in LPS-stimulated human umbilical vein endothelial cells (HUVECs) by monocyte adhesion and western blot assays. HUVECs were pretreated with 100 μg/ml TO for 1 h and then incubated with 1 μg/ml LPS for 24 h. The mRNA and protein expression levels of the targets (pro-inflammatory cytokines and adhesion molecules) were analyzed by real-time PCR and western blot assays. We also preformed HPLC analysis to identify the components of the TO methanol extract. The TO filtered methanol extracts dramatically inhibited LPS-induced endothelial cell–monocyte interactions by reducing vascular cell adhesion molecule-1 and monocyte chemoattractant protein-1, and pro-inflammatory cytokine expression. TO suppressed the LPS-induced nuclear translocation of NF-κB, whereas it did not affect MAPK activation. Our findings demonstrated that methanol extracts of TO could attenuate LPS-induced endothelial cell activation by inhibiting the NF-κB pathway. These results indicate the potential clinical benefits and applications of TO for the prevention of vascular inflammation and atherosclerosis. The online version of this article (10.1186/s12906-017-2022-7) contains supplementary material, which is available to authorized users.
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影响因子：
4.3
作者：
Jayashree, B.;Bibin, Y. S.;Balasubramanyam, M.
通讯作者：
Balasubramanyam, M.
影响因子：
--
作者：
Hu G;Wang J;Hong D;Zhang T;Duan H;Mu X;Yang Z
通讯作者：
Yang Z
动脉粥样硬化的炎症。
DOI：
10.1161/atvbaha.108.179705
发表时间：
2012-09
期刊：
Arteriosclerosis, thrombosis, and vascular biology
影响因子：
--
作者：
Libby P
通讯作者：
Libby P
影响因子：
2.4
作者：
Koh, Yoon-Jeoung;Cha, Dong-Soo;Choi, Hee-Don
通讯作者：
Choi, Hee-Don
影响因子：
37.8
作者：
Kiechl, S;Egger, G;Willeit, J
通讯作者：
Willeit, J