Molecular elements of pheromone detection in the female moth, Heliothis virescens

Molecular elements of pheromone detection in the female moth, Heliothis virescens
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雌蛾烟夜蛾信息素检测的分子元件

DOI:
10.1111/1744-7917.12434
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发表时间:
2017
期刊:
影响因子:
4
通讯作者:
Krieger J
Krieger J
中科院分区:
农林科学1区
文献类型:
--
作者:
Zielonka M;Breer H;Krieger J

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信息素在蛾类的生殖行为中起着关键作用,尤其是在雄蛾的求偶过程中。因此,检测雄蛾释放的雌蛾信息素的分子基础已经被非常详细地研究。相反,很少有人知道女性如何检测信息素成分释放自己或同种。在这项研究中,我们评估了触角的女性Heliothis virescensfor元素的信息素检测。根据先前的发现,雌性触角对性信息素成分(Z)-9-十四烯醛做出反应,我们鉴定了表达其同源受体(受体类型HR 6)的嗅觉感觉神经元。所有HR 6细胞共表达“感觉神经元膜蛋白1”(SNMP 1),并与表达信息素结合蛋白PBP 1和PBP 2的支持细胞相关。这些功能让人想起男性触角和点一致的机制,在两种性别的信息素检测。对SNMP 1表达细胞的进一步分析显示,与雄性相比,雌性中的SNMP 1表达细胞数量更高。此外,在雌性中,SNMP 1神经元排列成簇,将其树突投射到共同的感器中,而在雄性中只有单独的SNMP 1神经元,每个感器只有1个。并非所有的SNMP 1阳性细胞在雌性触角中表达HR 6,而是推定的信息素受体HR 11和HR 18,分别。表达3种受体类型之一的神经元被分配到不同的感受器。这些数据共同表明,在女性的触角,感觉神经元的一个子集的感觉器admindea配备了分子元素,使他们响应信息素。
Pheromones play pivotal roles in the reproductive behavior of moths, most prominently for the mate finding of male moths. Accordingly, the molecular basis for the detection of female‐released pheromones by male moths has been studied in great detail. In contrast, little is known about how females can detect pheromone components released by themselves or by conspecifics. In this study, we assessed the antenna of femaleHeliothis virescensfor elements of pheromone detection. In accordance with previous findings that female antennae respond to the sex pheromone component (Z)‐9‐tetradecenal, we identified olfactory sensory neurons that express its cognate receptor, the receptor type HR6. All HR6 cells coexpressed the “sensory neuron membrane protein 1” (SNMP1) and were associated with supporting cells expressing the pheromone‐binding proteins PBP1 and PBP2. These features are reminiscent to male antennae and point to congruent mechanisms for pheromone detection in the two sexes. Further analysis of the SNMP1‐expressing cells revealed a higher number in females compared to males. Moreover, in females, the SNMP1 neurons were arranged in clusters, which project their dendrites into a common sensillum, whereas in males there were only solitary SNMP1‐neurons and only 1 per sensillum. Not all SNMP1 positive cells in female antennae expressed HR6 but instead the putative pheromone receptors HR11 and HR18, respectively. Neurons expressing 1 of the 3 receptor types were assigned to different sensilla. Together the data indicate that on the antenna of females, sensory neurons in a subset of sensilla trichodea are equipped with molecular elements, which render them responsive to pheromones.
细胞神经科学前沿 未成熟苔藓纤维 Bers-ca3 连接的细胞神经科学,内源性释放的 Gaba 激活突触前 Gaba B 受体有助于突触沉默
DOI: --
发表时间: --
期刊:
影响因子: --
作者:
Victoria F Safi;E. Cherubini
通讯作者: E. Cherubini
斜纹夜蛾 OR18 的鉴定和表达谱,该基因在嗅觉识别中具有潜在作用
DOI: --
发表时间: 2014
期刊:
影响因子: --
作者:
Kaidi Zheng;Xinda Lin;Yongjun Du
通讯作者: Yongjun Du
DOI: 10.1111/imb.12119
发表时间: 2014-12
影响因子: 2.6
作者:
P. Pregitzer;M. Greschista;H. Breer;J. Krieger
通讯作者: P. Pregitzer;M. Greschista;H. Breer;J. Krieger
DOI: 10.1016/0965-1748(93)90052-t
发表时间: 1993-06-01
影响因子: 3.8
作者:
KRIEGER, J;GANSSLE, H;BREER, H
通讯作者: BREER, H
DOI: 10.1007/s00359-007-0220-3
发表时间: 2007-06-01
影响因子: 2.1
作者:
Hillier, N. K.;Vickers, N. J.
通讯作者: Vickers, N. J.