Preimplantation processing of ex vivo-derived vascular biomaterials: effects on peripheral cell adhesion.
Preimplantation processing of ex vivo-derived vascular biomaterials: effects on peripheral cell adhesion.
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DOI：
10.1002/jbm.a.34308
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发表时间：
2013-01
影响因子：
4.9
通讯作者：
McFetridge, Peter S.
中科院分区：
文献类型：
--
作者：
Uzarski, Joseph S.;Van de Walle, Aurore B.;McFetridge, Peter S.
使用离体衍生的支架作为血管导管已被证明是修复或绕过闭塞血管的临床有效方法。同种异体组织移植物的植入需要仔细处理以降低免疫原性并防止细菌感染。然而，用于制备生物支架的机械/化学处理可导致天然结构和表面化学的显著改变，这可影响体内性能。对于血管移植物特别重要的是植入的生物材料与来自循环和邻近脉管系统的宿主细胞之间的结合相互作用。在这里，我们提出了一个比较的四种策略，用于脱细胞异体人脐静脉（HUV）支架：乙醇/丙酮，氯化钠，十二烷基硫酸钠（SDS），或Triton X-100。扫描电子显微镜显示，所有四种技术都实现了从HUV移植物的内腔和外腔表面去除天然细胞。血小板和早幼粒细胞HL-60细胞表现出更松散的结构ablumenal表面上的优先结合，虽然低表面覆盖率观察到的外周血细胞。血管内皮细胞粘附在使用乙醇/丙酮的HUV脱细胞上最高，并且显著高于SDS处理的移植物（p = 0.016）。无论去细胞技术如何，原代细胞在管腔表面上显示出高活力（在所有情况下超过95%）。这些结果证明了各种化学处理策略对离体衍生血管移植物的粘附性能的关键影响。在选择最大限度地减少通常对移植物存活有害的先天性反应（例如血栓形成、炎症）的处理策略时，需要仔细考虑特定应用。
The use of ex vivo-derived scaffolds as vascular conduits has shown to be a clinically valid approach to repair or bypass occluded vessels. Implantation of allogeneic tissue grafts requires careful processing to lower immunogenicity and prevent bacterial infection. However, the mechanical/chemical treatments used to prepare biological scaffolds can result in significant alterations to the native structure and surface chemistry, which can affect in vivo performance. Of particular importance for vascular grafts are binding interactions between the implanted biomaterial and host cells from the circulation and adjacent vasculature. Here we present a comparison of four strategies used to decellularize allogeneic human umbilical vein (HUV) scaffolds: ethanol/acetone, sodium chloride, sodium dodecyl sulfate (SDS), or Triton X-100. Scanning electron microscopy revealed that all four techniques achieved removal of native cells from both the lumenal and ablumenal surfaces of HUV grafts. Platelets and promyelocytic HL-60 cells showed preferential binding on the more loosely structured ablumenal surface, although low surface coverage was observed overall by peripheral blood cells. Vascular endothelial cell adhesion was highest on HUV decellularized using ethanol/acetone, and significantly higher than on SDS-processed grafts (p = 0.016). Primary cells showed high viability on the lumenal surface regardless of decellularization technique (over 95% in all cases). These results demonstrate the critical effects of various chemical processing strategies on the adhesive properties of ex vivo-derived vascular grafts. Careful application-specific consideration is warranted when selecting a processing strategy that minimizes innate responses (e.g. thrombosis, inflammation) that are often deleterious to graft survival.
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影响因子：
--
作者：
Gratzer, Paul F.;Harrison, Robert D.;Woods, Terence
通讯作者：
Woods, Terence
影响因子：
4.3
作者：
Dardik, H;Wengerter, K;Ibrahim, IM
通讯作者：
Ibrahim, IM
影响因子：
15.9
作者：
JAFFE, EA;NACHMAN, RL;MINICK, CR
通讯作者：
MINICK, CR
影响因子：
4.3
作者：
Johnson, WC;Lee, KK
通讯作者：
Lee, KK
影响因子：
2.4
作者：
Abousleiman, Rita I.;Reyes, Yuliana;Sikavitsas, Vassilios
通讯作者：
Sikavitsas, Vassilios