Diverse MarR bacterial regulators of auxin catabolism in the plant microbiome.
Diverse MarR bacterial regulators of auxin catabolism in the plant microbiome.
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植物微生物组中生长素分解代谢的多种细菌调节剂。
DOI：
10.1038/s41564-022-01244-3
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发表时间：
2022-11
影响因子：
28.3
通讯作者：
中科院分区：
文献类型：
--
作者：
植物微生物组中的化学信号可以对微生物群落结构以及宿主生长和发育产生巨大影响。以前，我们证明了生长素代谢信号干扰细菌属Variovorax通过生长素降解位点是必不可少的，以维持刻板的根发育在生态相关的细菌合成社区。在这里，我们解剖Variovorax生长素降解位点定义的基因iadDE作为必要和充分的吲哚-3-乙酸（IAA）降解和信号干扰。我们确定的晶体结构和结合特性的操纵子的MarR家族阻遏物与IAA和其他生长素。生长素降解操纵子在细菌生命树中被鉴定，我们根据基因含量和代谢产物定义了两种不同的类型：iac样和iad样。来自每个生长素降解操纵子类型的代表的MarR的结构确定每个具有不同的IAA结合口袋。代表性的IAA降解菌株从不同的细菌属殖民拟南芥植物的比较表明，虽然所有降解IAA，只有含有iad样生长素降解操纵子的菌株干扰生长素信号在一个复杂的合成社区的背景下。这表明含有iad样操纵子的细菌菌株，包括Variovorax物种，在调节植物微生物组中的生长素方面发挥关键的生态作用。细菌生长素降解位点及其调节剂的表征揭示了植物微生物组物种中的两种不同类型，其中只有一种，例如Variovorax物种，可以干扰复杂合成微生物群落中的根生长抑制。
Chemical signalling in the plant microbiome can have drastic effects on microbial community structure, and on host growth and development. Previously, we demonstrated that the auxin metabolic signal interference performed by the bacterial genus Variovorax via an auxin degradation locus was essential for maintaining stereotypic root development in an ecologically relevant bacterial synthetic community. Here, we dissect the Variovorax auxin degradation locus to define the genes iadDE as necessary and sufficient for indole-3-acetic acid (IAA) degradation and signal interference. We determine the crystal structures and binding properties of the operon’s MarR-family repressor with IAA and other auxins. Auxin degradation operons were identified across the bacterial tree of life and we define two distinct types on the basis of gene content and metabolic products: iac-like and iad-like. The structures of MarRs from representatives of each auxin degradation operon type establish that each has distinct IAA-binding pockets. Comparison of representative IAA-degrading strains from diverse bacterial genera colonizing Arabidopsis plants show that while all degrade IAA, only strains containing iad-like auxin-degrading operons interfere with auxin signalling in a complex synthetic community context. This suggests that iad-like operon-containing bacterial strains, including Variovorax species, play a key ecological role in modulating auxins in the plant microbiome. Characterization of bacterial auxin degradation loci and their regulators reveals two distinct types across plant microbiome species, where only one, exemplified in Variovorax species, can interfere with root growth inhibition in a complex synthetic microbial community.
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影响因子：
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作者：
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通讯作者：
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