Deletion of MtrA Inhibits Cellular Development of Streptomyces coelicolor and Alters Expression of Developmental Regulatory Genes.
Deletion of MtrA Inhibits Cellular Development of Streptomyces coelicolor and Alters Expression of Developmental Regulatory Genes.
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DOI：
10.3389/fmicb.2017.02013
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发表时间：
2017
影响因子：
5.2
通讯作者：
Pang X
中科院分区：
文献类型：
--
作者：
Zhang P;Wu L;Zhu Y;Liu M;Wang Y;Cao G;Chen XL;Tao M;Pang X
链霉菌属的发育生命周期包括气生菌丝形成和孢子成熟，这两个不同的发育过程分别由两个家族的发育调控基因bld和whi控制。在这项研究中，我们表明，反应调节剂MtrA（SCO3013）是至关重要的气生菌丝在S。coelicolor和相关物种。ΔmtrA是反应调节基因mtrA的缺失突变体，表现出典型的气生菌丝体形成缺陷型突变体的秃表型，其菌丝体的形成或延迟或不形成取决于培养基。转录分析表明，MtrA激活参与气生菌丝体形成的多个基因，包括chp、rdl和ram基因，以及bld和whi家族的发育调控基因。然而，主要调控基因bldD在ΔmtrA中表现出增强的表达，表明其被MtrA抑制。电泳迁移率变动分析表明，MtrA结合在ΔmtrA中表达改变的几个基因的上游，包括bldD和whiI，并且在结合位点中发现了与另一种放线菌结核分枝杆菌MtrA的共有结合序列相似的序列。一个松散保守的识别序列，包含两个短的，直接重复序列被确定为MtrA的S。coelicolor，并使用突变分析进行验证。MtrA同源物广泛分布于链霉菌属中，与S.天蓝色，从S. venezuelae和sli_3357。变铅青导致条件性秃顶形态。我们的研究表明，MtrA在链霉菌的发展中起着关键和保守的作用。
The developmental life cycle of Streptomyces species includes aerial hyphae formation and spore maturation, two distinct developmental processes that are controlled, respectively, by two families of developmental regulatory genes, bld and whi. In this study, we show that the response regulator MtrA (SCO3013) is critical for normal development of aerial hyphae in S. coelicolor and related species. ΔmtrA, a deletion mutant of the response regulator gene mtrA, exhibited the bald phenotype typical of bld mutants defective in aerial mycelium formation, with formation either much delayed or absent depending on the culture medium. Transcriptional analysis indicated that MtrA activates multiple genes involved in formation of aerial mycelium, including chp, rdl, and ram genes, as well as developmental regulatory genes of the bld and whi families. However, the major regulatory gene bldD showed enhanced expression in ΔmtrA, suggesting it is repressed by MtrA. electrophoretic mobility shift assays indicated that MtrA binds upstream of several genes with altered expression in ΔmtrA, including bldD and whiI, and sequences similar to the consensus binding sequence for MtrA of another actinomycete, Mycobacterium tuberculosis, were found in the bound sites. A loosely conserved recognition sequence containing two short, direct repeats was identified for MtrA of S. coelicolor and was validated using mutational analysis. MtrA homologs are widely distributed among Streptomyces species, and as with S. coelicolor, deletion of the mtrA homologs sve_2757 from S. venezuelae and sli_3357 from S. lividans resulted in conditional bald morphology. Our study suggests a critical and conserved role for MtrA in Streptomyces development.
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影响因子：
6.4
作者：
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通讯作者：
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