High miR-21 expression from FFPE tissues is associated with poor survival and response to adjuvant chemotherapy in colon cancer.
High miR-21 expression from FFPE tissues is associated with poor survival and response to adjuvant chemotherapy in colon cancer.
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来自FFPE组织的高miR-21表达与结肠癌中辅助化疗的生存不良和对辅助化疗的反应有关。
DOI：
10.1002/ijc.28522
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发表时间：
2014-04-15
影响因子：
6.4
通讯作者：
Harris, Curtis C.
中科院分区：
文献类型：
--
作者：
Oue, Naohide;Anami, Katsuhiro;Schetter, Aaron J.;Moehler, Markus;Okayama, Hirokazu;Khan, Mohammed A.;Bowman, Elise D.;Mueller, Annett;Schad, Arno;Shimomura, Manabu;Hinoi, Takao;Aoyagi, Kazuhiko;Sasaki, Hiroki;Okajima, Masazumi;Ohdan, Hideki;Galle, Peter R.;Yasui, Wataru;Harris, Curtis C.
结肠癌（CC）是癌症死亡的主要原因。需要新的生物标志物来识别复发风险高的CC患者和可能从治疗干预中受益的患者。本研究的目的是研究从福尔马林固定石蜡包埋（FFPE）组织切片中分离的RNA中的miR-21表达是否与CC患者的预后和治疗结果相关。通过定量逆转录酶-聚合酶链反应测量日本队列（I-IV期，n = 156）和德国队列（II期，n = 145）中miR-21的表达。在II/III期日本（P = 0.0008）和II期德国（P = 0.047）队列中，肿瘤中miR-21的高表达与生存率差相关。在多变量模型中，这些关联独立于其他临床协变量。接受辅助化疗对任一队列中高miR-21的患者均无益处。在日本队列中，高miR-21表达与不良治疗结局显著相关（P = 0.0001），辅助治疗与低miR-21患者的生存率改善相关（P = 0.001）。这些结果表明，miR-21是一种有前途的生物标志物，可用于识别预后不良的患者，并可在FFPE组织中准确测量。miR-21的表达也可以识别将从辅助化疗中受益的患者。
Colon cancer (CC) is a leading cause of cancer mortality. Novel biomarkers are needed to identify CC patients at high risk of recurrence and those who may benefit from therapeutic intervention. The aim of this study is to investigate if miR-21 expression from RNA isolated from formalin-fixed paraffin-embedded (FFPE) tissue sections is associated with prognosis and therapeutic outcome for patients with CC. The expression of miR-21 was measured by quantitative reverse transcriptase-polymerase chain reaction in a Japanese cohort (stage I–IV, n = 156) and a German cohort (stage II, n = 145). High miR-21 expression in tumors was associated with poor survival in both the stage II/III Japanese (P = 0.0008) and stage II German (P = 0.047) cohorts. These associations were independent of other clinical covariates in multivariable models. Receipt of adjuvant chemotherapy was not beneficial in patients with high miR-21 in either cohort. In the Japanese cohort, high miR-21 expression was significantly associated with poor therapeutic outcome (P = 0.0001) and adjuvant therapy was associated with improved survival in patients with low miR-21 (P = 0.001). These results suggest that miR-21 is a promising biomarker to identify patients with poor prognosis and can be accurately measured in FFPE tissues. The expression of miR-21 may also identify patients who will benefit from adjuvant chemotherapy.
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影响因子：
64.8
作者：
Lu, J;Getz, G;Golub, TR
通讯作者：
Golub, TR
影响因子：
3.5
作者：
Goswami RS;Waldron L;Machado J;Cervigne NK;Xu W;Reis PP;Bailey DJ;Jurisica I;Crump MR;Kamel-Reid S
通讯作者：
Kamel-Reid S
影响因子：
5
作者：
Hui, Angela B. Y.;Shi, Wei;Liu, Fei-Fei
通讯作者：
Liu, Fei-Fei
影响因子：
20.3
作者：
Rossi, Simona;Shimizu, Masayoshi;Calin, George A.
通讯作者：
Calin, George A.
影响因子：
5.3
作者：
Krichevsky AM;Gabriely G
通讯作者：
Gabriely G