KIF23 enhances cell proliferation in pancreatic ductal adenocarcinoma and is a potent therapeutic target.
KIF23 enhances cell proliferation in pancreatic ductal adenocarcinoma and is a potent therapeutic target.
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DOI：
10.21037/atm-20-1970
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发表时间：
2020-11
影响因子：
--
通讯作者：
Zhou YZ
中科院分区：
文献类型：
--
作者：
Gao CT;Ren J;Yu J;Li SN;Guo XF;Zhou YZ
近年来的研究表明，作为参与胞质分裂调节的驱动蛋白马达蛋白之一，驱动蛋白家族成员23（KIF23）的高表达与胶质瘤和紫杉醇耐药胃癌的不良预后相关，其结果是增殖、迁移和侵袭的增强。在这项研究中，我们分析了KIF23在胰腺导管腺癌进展中的作用。采用生物信息学方法分析KIF23在胰腺癌组织和正常胰腺组织中的表达水平，并分析KIF23高表达与预后的关系。我们采用免疫组织化学方法检测KIF23的表达，并分析KIF23的表达与胰腺导管腺癌患者临床病理特征之间的关系。此外，在体外进行集落形成测定、MTT测定和蛋白质印迹测定，沿着在体内进行小鼠异种移植模型，以分析KIF 23对增殖的影响。进一步通过TCGA和免疫组化数据分析KIF23和CDCA8之间的相关性。生物信息学结果显示，KIF23 mRNA在胰腺肿瘤组织中的表达高于正常胰腺组织，并且预后不良与KIF23的高表达有关。免疫组化显示KIF23在蛋白水平上高表达，且KIF23的高表达与不良的临床病理特征相关。我们的实验结果表明，敲低KIF23可以抑制胰腺细胞的增殖。KIF23与CDCA8的表达呈正相关，KIF23可能通过影响CDCA8的表达促进胰腺癌的增殖。我们的数据表明KIF23的高表达与不良预后相关，并且KIF23可能是胰腺导管腺癌的潜在治疗靶点。
In recent research, high expression of kinesin family member 23 (KIF23), one of the kinesin motor proteins involved in the regulation of cytokinesis, has been shown to be related to poor prognosis in glioma and paclitaxel-resistant gastric cancer, as a results of the enhancement of proliferation, migration, and invasion. In this study, we analyzed the role of KIF23 in the progression of pancreatic ductal adenocarcinoma. A bioinformatic method was used to analyze the KIF23 mRNA level in pancreatic tumor tissues compared with normal pancreatic tissues and to analyze the connection between high KIF23 expression and prognosis. We examined the expression of KIF23 using immunohistochemistry and analyzed the connection between the expression of KIF23 and clinicopathological features in pancreatic ductal adenocarcinoma patients. In addition, a colony formation assay, MTT assay, and western blot assay were performed in vitro, along with a mouse xenograft model in vivo, to analyze the effect of KIF23 on proliferation. Further, the correlation between KIF23 and CDCA8 was analyzed by TCGA and immunohistochemical data. Bioinformatic results showed that KIF23 mRNA expression was higher in pancreatic tumor tissues than in normal pancreatic tissues and a poor prognosis has been linked to the high expression of KIF23. Immunohistochemistry revealed that KIF23 was highly expressed at the protein level and high expression of KIF23 correlated with adverse clinicopathological features. Our experimental results demonstrated that knockdown of KIF23 could inhibit the proliferation of pancreatic cells. Further, a positive correlation between KIF23 and CDCA8 expression existed, and KIF23 might promote pancreatic cancer proliferation by affecting CDCA8 expression. Our data showed that high expression of KIF23 is associated with a poor prognosis, and KIF23 might be a potential therapeutic target for pancreatic ductal adenocarcinoma.
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影响因子：
14.9
作者：
Schmidt EE;Pelz O;Buhlmann S;Kerr G;Horn T;Boutros M
通讯作者：
Boutros M
DOI：
10.1083/jcb.200109090
发表时间：
2002-03-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kuriyama R;Gustus C;Terada Y;Uetake Y;Matuliene J
通讯作者：
Matuliene J
影响因子：
4.3
作者：
Rossi, Maria L.;Rehman, Azeem A.;Gondi, Christopher S.
通讯作者：
Gondi, Christopher S.
影响因子：
64.8
作者：
NISLOW, C;LOMBILLO, VA;MCINTOSH, JR
通讯作者：
MCINTOSH, JR
影响因子：
7.5
作者：
Ayhan, Ayse;Kuhn, Elisabetta;Wang, Tian-Li
通讯作者：
Wang, Tian-Li