CHO1, a mammalian kinesin-like protein, interacts with F-actin and is involved in the terminal phase of cytokinesis.
CHO1, a mammalian kinesin-like protein, interacts with F-actin and is involved in the terminal phase of cytokinesis.
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DOI：
10.1083/jcb.200109090
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发表时间：
2002-03-04
期刊：
影响因子：
--
通讯作者：
Matuliene J
中科院分区：
文献类型：
--
作者：
Kuriyama R;Gustus C;Terada Y;Uetake Y;Matuliene J
CHO1是有丝分裂运动蛋白（MKLP）1亚家族中的一种运动蛋白样蛋白，存在于哺乳动物细胞的中央纺锤体和中间体中。与其他亚家族成员不同的是，它在cooh末端尾部含有一个额外的~ 300 bp。鸡基因组序列分析表明，异质性来源于选择性剪接，外显子18只表达CHO1亚型。CHO1及其截断的同种异构体MKLP1在单个细胞中共表达。令人惊讶的是，外显子18编码的序列具有与f -肌动蛋白相互作用的能力，这表明CHO1可以与微管和肌动蛋白细胞骨架结合。显微注射外显子18特异性抗体对核分裂和细胞分裂早期没有任何抑制作用。然而，几乎完全分离的子细胞重新结合形成双管状细胞，这表明外显子18蛋白可能在皮层收缩环的形成和进入中没有作用。相反，它可能直接或间接参与完成细胞质分裂末期所必需的膜事件。
CHO1 is a kinesin-like protein of the mitotic kinesin-like protein (MKLP)1 subfamily present in central spindles and midbodies in mammalian cells. It is different from other subfamily members in that it contains an extra ∼300 bp in the COOH-terminal tail. Analysis of the chicken genomic sequence showed that heterogeneity is derived from alternative splicing, and exon 18 is expressed in only the CHO1 isoform. CHO1 and its truncated isoform MKLP1 are coexpressed in a single cell. Surprisingly, the sequence encoded by exon 18 possesses a capability to interact with F-actin, suggesting that CHO1 can associate with both microtubule and actin cytoskeletons. Microinjection of exon 18–specific antibodies did not result in any inhibitory effects on karyokinesis and early stages of cytokinesis. However, almost completely separated daughter cells became reunited to form a binulceate cell, suggesting that the exon 18 protein may not have a role in the formation and ingression of the contractile ring in the cortex. Rather, it might be involved directly or indirectly in the membrane events necessary for completion of the terminal phase of cytokinesis.
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影响因子：
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作者：
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