High-fat diet suppresses the positive effect of creatine supplementation on skeletal muscle function by reducing protein expression of IGF-PI3K-AKT-mTOR pathway.
High-fat diet suppresses the positive effect of creatine supplementation on skeletal muscle function by reducing protein expression of IGF-PI3K-AKT-mTOR pathway.
复制标题
DOI：
10.1371/journal.pone.0199728
复制
发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Mosqueira M
中科院分区：
文献类型：
--
作者：
Ferretti R;Moura EG;Dos Santos VC;Caldeira EJ;Conte M;Matsumura CY;Pertille A;Mosqueira M
高脂肪（HF）饮食与久坐不动的生活方式相结合，代表了一个主要的公共卫生问题，易患肥胖症和糖尿病，导致骨骼肌萎缩，纤维直径和肌肉质量减少，脂肪组织积累，导致肌肉力量的损失。克服HF饮食的不良影响的一种策略是阻力训练，这是一种用于改善肌肉质量的策略，恢复对骨骼肌中肥胖相关变化的负面影响。与阻力训练一起，在饮食中补充肌酸一水合物（CrM）已被用于改善肌肉质量和力量。肌酸是一种非必需氨基酸，直接参与跨桥循环，在肌肉收缩开始期间为ADP提供磷酸基团。除了它的抗氧化和抗炎作用CrM还上调IGF-1导致hypothophy与肌肉功能的增加。然而，目前尚不清楚在阻力训练期间补充CrM是否会逆转高脂肪饮食对肌肉性能的负面影响。在8周期间，我们测量了接受标准饮食或高脂肪饮食，补充或不补充CrM的训练大鼠在增加负荷的情况下爬1.1m和80°梯子的肌肉性能。我们观察到CrM补充上调IGF-1和磷酸化AKT蛋白水平，表明IGF 1-PI 3 K-Akt/PKB-mTOR通路的激活。此外，尽管补充了CrM，HF饮食下调了IGF 1-PI 3 K-Akt/PKB-mTOR通路的几种蛋白质，表明饮食脂质含量对维持或改善阻力训练期间的肌肉功能至关重要。
High-fat (HF) diets in combination with sedentary lifestyle represent one of the major public health concerns predisposing to obesity and diabetes leading to skeletal muscle atrophy, decreased fiber diameter and muscle mass with accumulation of fat tissue resulting in loss of muscle strength. One strategy to overcome the maleficent effects of HF diet is resistance training, a strategy used to improve muscle mass, reverting the negative effects on obesity-related changes in skeletal muscle. Together with resistance training, supplementation with creatine monohydrate (CrM) in the diet has been used to improve muscle mass and strength. Creatine is a non-essential amino acid that is directly involved in the cross-bridge cycle providing a phosphate group to ADP during the initiation of muscle contraction. Besides its antioxidant and anti-inflammatory effects CrM also upregulates IGF-1 resulting in hyperthophy with an increase in muscle function. However, it is unknown whether CrM supplementation during resistance training would revert the negative effects of high-fat diet on the muscle performance. During 8 weeks we measured muscle performance to climb a 1.1m and 80° ladder with increasing load on trained rats that had received standard diet or high-fat diet, supplemented or not with CrM. We observed that the CrM supplementation up-regulated IGF-1 and phospho-AKT protein levels, suggesting an activation of the IGF1-PI3K-Akt/PKB-mTOR pathway. Moreover, despite the CrM supplementation, HF diet down-regulated several proteins of the IGF1-PI3K-Akt/PKB-mTOR pathway, suggesting that diet lipid content is crucial to maintain or improve muscle function during resistance training.
登录
查看更多内容
影响因子：
2.4
作者：
Chilibeck PD;Kaviani M;Candow DG;Zello GA
通讯作者：
Zello GA
影响因子：
4.9
作者：
Bentzinger CF;Lin S;Romanino K;Castets P;Guridi M;Summermatter S;Handschin C;Tintignac LA;Hall MN;Rüegg MA
通讯作者：
Rüegg MA
影响因子：
3.5
作者：
Louis, M;Van Beneden, R;Francaux, M
通讯作者：
Francaux, M
影响因子：
5.5
作者：
Deldicque, Louise;Theisen, Daniel;Francaux, Marc
通讯作者：
Francaux, Marc
影响因子：
3.3
作者：
Kirwan, JP;Del Aguila, LF;Krishnan, RK
通讯作者：
Krishnan, RK