Deep mRNA sequencing reveals stage-specific transcriptome alterations during microsclerotia development in the smoke tree vascular wilt pathogen, Verticillium dahliae.
Deep mRNA sequencing reveals stage-specific transcriptome alterations during microsclerotia development in the smoke tree vascular wilt pathogen, Verticillium dahliae.
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深度 mRNA 测序揭示了烟树维管枯萎病病原体大丽黄萎病菌微菌核发育过程中阶段特异性转录组变化
DOI：
10.1186/1471-2164-15-324
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发表时间：
2014-05-01
期刊：
影响因子：
4.4
通讯作者：
Tian C
中科院分区：
文献类型：
--
作者：
Xiong D;Wang Y;Ma J;Klosterman SJ;Xiao S;Tian C
大丽轮枝菌（Verticillium dahliae）是一种土传真菌，能在土壤中存活数年，产生多细胞、黑化的休眠体，也称为微菌核（microsclerotia，MS）。MS是轮枝菌病循环的主要感染源。因此，MS的形成标志着V的疾病周期中的重要事件。结果利用新一代测序技术RNA-Seq，研究了MS形成过程中的转录组学动态变化，确定了MS形成过程中不同阶段的基因表达差异。大丽花，从烟树分离。我们观察到MS形成过程中基因表达的大规模变化，例如参与蛋白质代谢和碳水化合物代谢的基因表达增加。参与糖酵解途径和黑色素生物合成的基因在MS中显着上调。聚类分析显示，在整个MS发育过程中，参与初级代谢和应激反应的编码产物的基因表达增加。在MS的发展过程中，泛素依赖的蛋白catenin和细胞死亡相关基因的差异表达被揭示。位于V的谱系特异性（LS）区域的基因同源物。17）不表达或低表达。此外，选择性剪接（AS）事件进行了分析，揭示了超过95.0%的AS事件涉及保留的内含子（RI）。结论这些数据揭示了MS形成过程中的转录调控的动态，并被用来构建一个全面的高分辨率基因表达图谱。该图谱为理解V的MS发展的生物学和分子基础提供了关键资源。大丽花
BackgroundVerticillium dahliaeis a soil-borne fungus that causes vascular wilt diseases in a wide range of plant hosts.V. dahliaeproduces multicelled, melanized resting bodies, also known as microsclerotia (MS) that can survive for years in the soil. The MS are the primary source of infection of the Verticillium disease cycle. Thus, MS formation marks an important event in the disease cycle ofV. dahliae.ResultsIn this study, next generation sequencing technology of RNA-Seq was employed to investigate the global transcriptomic dynamics of MS development to identify differential gene expression at several stages of MS formation in strain XS11 ofV. dahliae,isolated from smoke tree. We observed large-scale changes in gene expression during MS formation, such as increased expression of genes involved in protein metabolism and carbohydrate metabolism. Genes involved in glycolytic pathway and melanin biosynthesis were dramatically up-regulated in MS. Cluster analyses revealed increased expression of genes encoding products involved in primary metabolism and stress responses throughout MS development. Differential expression of ubiquitin-dependent protein catabolism and cell death-associated genes during MS development were revealed. Homologs of genes located in the lineage-specific (LS) regions ofV. dahliaestrain VdLs.17, were either not expressed or showed low expression. Furthermore, alternative splicing (AS) events were analyzed, revealing that over 95.0% AS events involve retention of introns (RI).ConclusionsThese data reveal the dynamics of transcriptional regulation during MS formation and were used to construct a comprehensive high-resolution gene expression map. This map provides a key resource for understanding the biology and molecular basis of MS development ofV. dahliae.
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DOI：
10.1007/bf00408881
发表时间：
1970-01-01
期刊：
ARCHIV FUR MIKROBIOLOGIE
影响因子：
--
作者：
GRIFFITHS, DA
通讯作者：
GRIFFITHS, DA
影响因子：
2.8
作者：
GRIFFITHS, DA
通讯作者：
GRIFFITHS, DA
影响因子：
64.8
作者：
Graveley BR;Brooks AN;Carlson JW;Duff MO;Landolin JM;Yang L;Artieri CG;van Baren MJ;Boley N;Booth BW;Brown JB;Cherbas L;Davis CA;Dobin A;Li R;Lin W;Malone JH;Mattiuzzo NR;Miller D;Sturgill D;Tuch BB;Zaleski C;Zhang D;Blanchette M;Dudoit S;Eads B;Green RE;Hammonds A;Jiang L;Kapranov P;Langton L;Perrimon N;Sandler JE;Wan KH;Willingham A;Zhang Y;Zou Y;Andrews J;Bickel PJ;Brenner SE;Brent MR;Cherbas P;Gingeras TR;Hoskins RA;Kaufman TC;Oliver B;Celniker SE
通讯作者：
Celniker SE
影响因子：
4.4
作者：
Duressa D;Anchieta A;Chen D;Klimes A;Garcia-Pedrajas MD;Dobinson KF;Klosterman SJ
通讯作者：
Klosterman SJ
影响因子：
3.2
作者：
HAWKE, MA;LAZAROVITS, G
通讯作者：
LAZAROVITS, G