Mechanical Way To Study Molecular Structure of Pericellular Layer.
Mechanical Way To Study Molecular Structure of Pericellular Layer.
复制标题
DOI：
10.1021/acsami.3c06341
复制
发表时间：
2023-08-02
影响因子：
9.5
通讯作者：
Sokolov, Igor
中科院分区：
文献类型：
--
作者：
Makarova, Nadezda;Lekka, Malgorzata;Gnanachandran, Kajangi;Sokolov, Igor
关键词：
原子力显微镜（AFM）已被用于研究细胞，特别是恶性细胞的机械性能。已经报道了各种细胞类型的各种癌细胞与其非恶性对应物相比的软化。然而，在大多数AFM研究中，细胞周层被忽略。如图所示，它可能会大大改变测量的细胞刚度和错过的重要信息的物理性质的细胞周层。在这里，我们考虑到细胞周围层，使用刷子模型做AFM压痕研究膀胱上皮细胞膀胱非恶性（HCV 29）和癌（TCC29）细胞。它使我们能够测量不仅准静态杨氏模量的细胞体，但也的物理性质的细胞周围层（平衡长度和接枝密度）。我们发现癌细胞的内部细胞周刷较长，但其移植密度与非恶性细胞相似。对于癌细胞来说，外刷要短得多，密度也低得多。此外，我们展示了一种方法，将所获得的物理性质的细胞周围层的生化语言更好地了解细胞生物学社区。它是通过使用肝素酶I和神经氨酸酶酶处理，去除细胞周层的特定分子部分。这里提出的方法也可以用来破译不仅是细胞周围的分子组成，但也其他分子层。
Atomic force microscopy (AFM) has been used to study the mechanical properties of cells, in particular, malignant cells. Softening of various cancer cells compared to their nonmalignant counterparts has been reported for various cell types. However, in most AFM studies, the pericellular layer was ignored. As was shown, it could substantially change the measured cell rigidity and miss important information on the physical properties of the pericellular layer. Here we take into account the pericellular layer by using the brush model to do the AFM indentation study of bladder epithelial bladder nonmalignant (HCV29) and cancerous (TCCSUP) cells. It allows us to measure not only the quasistatic Young’s modulus of the cell body but also the physical properties of the pericellular layer (the equilibrium length and grafting density). We found that the inner pericellular brush was longer for cancer cells, but its grafting density was similar to that found for nonmalignant cells. The outer brush was much shorter and less dense for cancer cells. Furthermore, we demonstrate a method to convert the obtained physical properties of the pericellular layer into biochemical language better known to the cell biology community. It is done by using heparinase I and neuraminidase enzymatic treatments that remove specific molecular parts of the pericellular layer. The presented here approach can also be used to decipher the molecular composition of not only pericellular but also other molecular layers.
登录
查看更多内容
影响因子：
3.5
作者：
Gavara N;Chadwick RS
通讯作者：
Chadwick RS
影响因子：
3.5
作者：
Berdyyeva, TK;Woodworth, CD;Sokolov, I
通讯作者：
Sokolov, I
影响因子：
3.4
作者：
Dokukin, Maxim;Ablaeva, Yulija;Sokolov, Igor
通讯作者：
Sokolov, Igor
影响因子：
5.6
作者：
Alhuthali S;Kotidis P;Kontoravdi C
通讯作者：
Kontoravdi C
影响因子：
38.3
作者：
通讯作者：
--