Atomic force microscopy detects differences in the surface brush of normal and cancerous cells.
Atomic force microscopy detects differences in the surface brush of normal and cancerous cells.
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DOI：
10.1038/nnano.2009.77
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发表时间：
2009-06
影响因子：
38.3
通讯作者：
中科院分区：
文献类型：
--
作者：
原子力显微镜被广泛用于研究细胞的形态，但它也可以探测细胞的力学。现在已知癌细胞可能具有与正常细胞不同的机械特性，但对这些差异的原因知之甚少。在这里，我们定量报告之间的差异正常和癌变的人宫颈上皮细胞考虑刷层的细胞表面。这些刷状层主要由微绒毛、微脊和纤毛组成，对于与环境的相互作用非常重要。根据软细胞上的“刷”模型对体外细胞获得的变形力曲线进行处理。我们发现，正常细胞的刷子具有一个长度，而癌细胞显示长刷和短刷，具有显着不同的密度。观察到的差异表明，刷层应考虑到细胞表面的机械手段时，表征。
The atomic force microscope is broadly used to study the morphology of cells but it can also probe the mechanics of cells. It is now known that cancerous cells may have different mechanical properties than normal cells but the reasons for these differences are poorly understood. Here we report quantitatively the differences between normal and cancerous human cervical epithelial cells by considering the brush layer on the cell surface. These brush layers, which consist mostly of microvilli, microridges, and cilia are important for interacting with the environment. Deformation force curves obtained from cells in vitro are processed according to the 'brush on soft cell model. We found that normal cells have brushes with one length while cancerous cells displayed long and short brushes with significantly different densities. The observed differences suggest that brush layers should be taken into account when characterizing the cell surface by mechanical means.
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影响因子：
3.5
作者：
Berdyyeva, TK;Woodworth, CD;Sokolov, I
通讯作者：
Sokolov, I
影响因子：
2.2
作者：
Berdyyeva, T;Woodworth, CD;Sokolov, I
通讯作者：
Sokolov, I
DOI：
10.1016/j.nano.2005.12.002
发表时间：
2006-03-01
影响因子：
5.4
作者：
Sokolov, Igor;Iyer, Swaminathan;Woodworth, Craig D.
通讯作者：
Woodworth, Craig D.
影响因子：
4.4
作者：
Emerson, RJ;Camesano, TA
通讯作者：
Camesano, TA
影响因子：
64.5
作者：
Pugacheva, Elena N.;Jablonski, Sandra A.;Golemis, Erica A.
通讯作者：
Golemis, Erica A.