OSD1 promotes meiotic progression via APC/C inhibition and forms a regulatory network with TDM and CYCA1;2/TAM.
OSD1 promotes meiotic progression via APC/C inhibition and forms a regulatory network with TDM and CYCA1;2/TAM.
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DOI：
10.1371/journal.pgen.1002865
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发表时间：
2012
期刊：
影响因子：
4.5
通讯作者：
Mercier R
中科院分区：
文献类型：
--
作者：
Cromer L;Heyman J;Touati S;Harashima H;Araou E;Girard C;Horlow C;Wassmann K;Schnittger A;De Veylder L;Mercier R
与有丝分裂相比，细胞周期控制在减数分裂时被修改，因为两次分裂遵循单个DNA复制事件。细胞周期蛋白依赖性激酶（CDK）通过减数分裂和有丝分裂促进进展，并且其活性的中心调节剂是APC/C（后期促进复合物/环体），其对于退出有丝分裂特别需要。我们以前已经表明，OSD 1参与进入减数分裂I和减数分裂II在拟南芥，然而，OSD 1控制这些转换的分子机制仍然不清楚。在这里，我们表明，OSD 1通过APC/C抑制促进减数分裂进程。接下来，我们探索了OSD 1和已知控制拟南芥减数分裂细胞周期转换的基因之间的功能关系。如osd 1、cyca 1; 2/tam突变导致在第一次分裂后过早退出减数分裂，而tdm突变体在正常减数分裂I和II后进行异常的第三次减数分裂。值得注意的是，tdm对tam是上位的，osd 1对tdm是上位的。我们进一步表明，一个非破坏性CYCA 1;2/TAM的表达挑起，像TDM，进入第三次减数分裂。最后，我们发现CYCA 1;2/TAM与CDKA;1形成了一个活性复合物，可以在体外磷酸化OSD 1。因此，我们提出，一个功能网络组成的OSD 1，CYCA 1;2/TAM，和TDM控制减数分裂进程的三个关键步骤，其中OSD 1是一个减数分裂APC/C抑制剂。在有性生物的生命周期中，一个专门的细胞分裂-减数分裂-将染色体数目从两组（2n，二倍体）减少到一组（n，单倍体），而受精则恢复了原始的染色体数目。减数分裂减少倍性，因为它包括两个细胞分裂后，一个单一的DNA复制。在这项研究中，我们分析了一组基因的功能，共同控制进入第一次减数分裂，进入第二次减数分裂，并退出减数分裂的模式植物拟南芥。我们揭示了一个控制这三个关键转变的复杂调控网络。
Cell cycle control is modified at meiosis compared to mitosis, because two divisions follow a single DNA replication event. Cyclin-dependent kinases (CDKs) promote progression through both meiosis and mitosis, and a central regulator of their activity is the APC/C (Anaphase Promoting Complex/Cyclosome) that is especially required for exit from mitosis. We have shown previously that OSD1 is involved in entry into both meiosis I and meiosis II in Arabidopsis thaliana; however, the molecular mechanism by which OSD1 controls these transitions has remained unclear. Here we show that OSD1 promotes meiotic progression through APC/C inhibition. Next, we explored the functional relationships between OSD1 and the genes known to control meiotic cell cycle transitions in Arabidopsis. Like osd1, cyca1;2/tam mutation leads to a premature exit from meiosis after the first division, while tdm mutants perform an aberrant third meiotic division after normal meiosis I and II. Remarkably, while tdm is epistatic to tam, osd1 is epistatic to tdm. We further show that the expression of a non-destructible CYCA1;2/TAM provokes, like tdm, the entry into a third meiotic division. Finally, we show that CYCA1;2/TAM forms an active complex with CDKA;1 that can phosphorylate OSD1 in vitro. We thus propose that a functional network composed of OSD1, CYCA1;2/TAM, and TDM controls three key steps of meiotic progression, in which OSD1 is a meiotic APC/C inhibitor. In the life cycle of sexual organisms, a specialized cell division—meiosis—reduces the number of chromosomes from two sets (2n, diploid) to one set (n, haploid), while fertilization restores the original chromosome number. Meiosis reduces ploidy because it consists of two cellular divisions following a single DNA replication. In this study, we analyze the function of a group of genes that collectively controls the entry into the first meiotic division, the entry into the second meiotic division, and the exit from meiosis in the model plant Arabidopsis thaliana. We revealed a complex regulation network that controls these three key transitions.
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影响因子：
11.8
作者：
Isoda, Michitaka;Sako, Kosuke;Sagata, Noriyuki
通讯作者：
Sagata, Noriyuki
影响因子：
2.9
作者：
Busso, D;Delagoutte-Busso, B;Moras, D
通讯作者：
Moras, D
影响因子：
2.6
作者：
HORVATH, A;RIEZMAN, H
通讯作者：
RIEZMAN, H
影响因子：
14.9
作者：
Biegert A;Mayer C;Remmert M;Söding J;Lupas AN
通讯作者：
Lupas AN
影响因子：
4.5
作者：
d'Erfurth I;Cromer L;Jolivet S;Girard C;Horlow C;Sun Y;To JP;Berchowitz LE;Copenhaver GP;Mercier R
通讯作者：
Mercier R