Mechanism of the hairpin folding transformation of thymine-cytosine-rich oligonucleotides induced by Hg(II) and Ag(I) ions
Mechanism of the hairpin folding transformation of thymine-cytosine-rich oligonucleotides induced by Hg(II) and Ag(I) ions
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Hg(II)和Ag(I)离子诱导富含胸腺嘧啶胞嘧啶寡核苷酸发夹折叠转化的机制
DOI：
10.1140/epje/i2013-13101-5
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发表时间：
2013-09
影响因子：
1.8
通讯作者：
Liang, Haojun
中科院分区：
文献类型：
--
作者：
Ding, Wei;Xu, Mengze;Zhu, Hong;Liang, Haojun
通过等温滴定量热法、二级结构分析、平衡滴定和荧光研究，对金属诱导的富含胸腺嘧啶-胞嘧啶的寡核苷酸折叠成发夹状结构进行了表征。我们发现设计的富含胸腺嘧啶-胞嘧啶的寡核苷酸可以特异性地与Hg（II）或Ag（I）离子结合，以从无规卷曲结构产生发夹状结构中的金属介导的碱基对。通过等温滴定量热实验，详细研究了整个结合过程。热力学结果显示出两种可能的途径显着的变化后，加入汞（II）离子。此外，这种转化可以通过Ag（I）离子的存在而增强。通过荧光团和猝灭剂之间的荧光共振能量转移（FRET），荧光降低证实了发夹状结构的形成过程。光滴定数据分析表明，Hg（II）和Ag（I）离子的饱和结合化学计量比分别为12：1和4：1。我们的结果为研究金属介导的碱基对影响寡核苷酸结构转变的机制提供了一个有前途的策略，这可能最终导致构建金属触发的DNA折纸系统和含金属的DNA纳米技术的进展。
The metal-induced folding of thymine-cytosine-rich oligonucleotides into hairpin-like structures was characterised by isothermal titration calorimetry, secondary structure analysis, equilibrium titrations, and fluorescence study. We find that designed thymine-cytosine-rich oligonucleotides can specifically bind with Hg(II) or Ag(I) ions to generate metal-mediated base pairs in a hairpin-like structure from a random coil structure. Isothermal titration calorimetry experiments were performed to reveal the detail of the whole binding process. The thermodynamic result exhibits two possible pathways of significant change upon the addition of Hg(II) ions. Furthermore, this transformation can be enhanced by the presence of Ag(I) ions. The fluorescence decreases through fluorescence resonance energy transfer (FRET) between the fluorophore and quencher confirms the process of formation of the hairpin-like structure. The analysis of optical titration data demonstrates that the saturated binding stoichiometries are 12:1 and 4:1 for Hg(II) and Ag(I) ions, respectively. Our result provides a promising strategy for the investigation of the mechanism of structural transformation of oligonucleotides influenced by metal-mediated base pairs, which may eventually lead to progress in constructing a metal-triggered DNA origami system and metal-containing DNA nanotechnology.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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