Evaluation of accessory cell heterogeneity. I. Differential accessory cell requirement for T helper cell activation and for T‐B cooperation
Evaluation of accessory cell heterogeneity. I. Differential accessory cell requirement for T helper cell activation and for T‐B cooperation
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辅助细胞异质性的评估 I. T 辅助细胞激活和 T-B 合作的差异辅助细胞需求。
DOI：
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发表时间：
1985
影响因子：
5.4
通讯作者：
P. Erb
中科院分区：
文献类型：
--
作者：
G. Ramila;Susanne Studer;M. Kennedy;I. Sklenar;P. Erb
几种Ia+肿瘤细胞系和腹膜渗出巨噬细胞作为辅助细胞（AC）进行了抗原特异性T细胞激活和T - B协同作用的测试。巨噬细胞和所有Ia+肿瘤细胞系均诱导T细胞以主要组织相容性复合体（MHC）限制的方式释放淋巴因子，并重建AC -贫脾细胞或纯化T细胞和B细胞的抗体反应。然而，只有正常的巨噬细胞，而没有肿瘤系诱导载体特异性T辅助细胞（Th），通过连锁识别帮助B细胞特异性抗半抗原抗体反应。对于T - B协同作用，还需要辅助细胞，但与T细胞激活相比，任何类型的Ia+ AC（例如巨噬细胞或肿瘤系）都是有效的。如果将抗原脉冲AC加入到AC缺失的T - B合作培养中，可以观察到淋巴细胞和AC之间强烈的MHC限制。如果AC和抗原同时加入到AC -缺失的T - B培养中，MHC -限制不太明显。在抗原特异性Th细胞和半抗原载体偶联物存在的情况下，刀豆蛋白A上清重建了AC缺失T - B培养的反应。然而，如果加入肿瘤系激活的T细胞而不是巨噬细胞诱导的Th细胞，即使存在Con A上清液，也不会与B细胞发生合作。所得结果表明，根据Th细胞的分化阶段，诱导Th细胞的AC需求是不同的。
Several Ia+ tumor cell lines and peritoneal exudate macrophages were tested as accessory cells (AC) for the activation of antigen‐specific T cells and for T‐B cooperation. The macrophages and all the Ia+ tumor lines tested induced the release of lymphokines from T cells in a major histocompatibility complex (MHC)‐restricted fashion and reconstituted the antibody responses of AC‐depleted spleen cells or of purified T and B cells. However, only the normal macrophages but none of the tumor lines induced carrier‐specific T helper (Th) cells which help B cells for specific anti‐hapten antibody responses by linked recognition. For T‐B cooperation accessory cells were also required, but in contrast to Th Cell activation any type of Ia+ AC (e.g. macrophage or tumor line) was effective. Strong MHC‐restriction between the lymphocytes and the AC was seen if antigen‐pulsed AC were added into the AC‐depleted T‐B cooperation cultures. If the AC and antigen were concomitantly added to the AC‐depleted T‐B cultures, MHC‐restriction was less obvious. Concanavalin A supernatant reconstituted the response of AC‐depleted T‐B cultures provided antigen‐specific Th cells and the hapten‐carrier conjugate were present. If, however, tumor line‐activated T cells were added instead of macrophage‐induced Th cells, no cooperation with B cells took place even in the presence of Con A supernatant. The results obtained demonstrate a differential AC requirement for the induction of Th cells depending on the differentiation stage of the Th cells.
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影响因子：
4.4
作者：
R. Chesnut;H. Grey
通讯作者：
R. Chesnut;H. Grey
辅助细胞替代测定中肿瘤细胞系的表征和功能特性。
DOI：
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发表时间：
1982
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Walker,EB;Lanier,LL;Warner,NL
通讯作者：
Warner,NL
T 细胞有助于细胞毒性 T 淋巴细胞反应。
影响因子：
6.2
作者：
Livnat,S;Corley,RB
通讯作者：
Corley,RB
产生溶细胞性 T 淋巴细胞反应中的细胞相互作用：Ia 阳性脾贴壁细胞在 H-2 抗原呈递中的作用。
DOI：
10.1073/pnas.77.10.6091
发表时间：
1980
影响因子：
11.1
作者：
Weinberger,O;Herrmann,SH;Mescher,MF;Benacerraf,B;Burakoff,SJ
通讯作者：
Burakoff,SJ
增殖和辅助 T 淋巴细胞对人纤维蛋白肽 B 表现出独特的精细特异性。
DOI：
--
发表时间：
1983
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Peterson,LB;Wilner,GD;Thomas,DW
通讯作者：
Thomas,DW