The role of DNA (de)methylation in immune responsiveness of Arabidopsis.
The role of DNA (de)methylation in immune responsiveness of Arabidopsis.
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DOI：
10.1111/tpj.13252
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发表时间：
2016-11
期刊：
影响因子：
--
通讯作者：
Ton J
中科院分区：
文献类型：
--
作者：
López Sánchez A;Stassen JH;Furci L;Smith LM;Ton J
DNA 甲基化受到 DNA 甲基转移酶和 DNA 去甲基化酶的拮抗控制。 DNA 甲基化水平在整体水平上控制植物基因表达。我们研究了 DNA 甲基化的整体变化对拟南芥免疫系统的影响。一系列低甲基化突变体对生物营养病原体拟南芥子菌 (Hpa) 表现出增强的抗性，而两个高甲基化突变体对这种病原体更敏感。对低甲基化 nrpe1 突变体（RNA 指导的 DNA 甲基化受损）和高甲基化 ros1 突变体（DNA 去甲基化受影响）的后续表征表明，它们相反的耐药表型与细胞壁防御和水杨酸 (SA) 依赖性基因表达的变化有关。 nrpe1 对坏死性病原体黄瓜圆球菌感染的敏感性增强，这与茉莉酸 (JA) 诱导基因表达的敏感性受到抑制有关。相反，ros1 对坏死性病原体表现出增强的抵抗力，这与 JA 诱导基因表达的反应性增强无关。尽管 nrpe1 和 ros1 在对 Hpa 的系统获得性抗性中未受影响，但它们未能发展出针对该病原体的跨代获得性抗性。 Hpa 感染后多个时间点的 nrpe1 和 ros1 全局转录组分析表明，49% 的发病机制相关转录组受到 NRPE1 和 ROS1 控制的 DNA 甲基化的影响。在 166 个防御相关基因中，nrpe1 的诱导增强，ros1 的诱导抑制，只有 25 个基因与附近的转座元件和 NRPE1 和/或 ROS1 控制的 DNA 甲基化相关。因此，我们认为大多数 NRPE1 和 ROS1 依赖性防御基因受到 DNA 甲基化的反式调节。 DNA 甲基化与防御启动有关，但尚不完全了解哪些类型的植物免疫受到影响或 DNA 甲基化参与的程度如何。我们通过对 DNA 甲基化受到相反影响的拟南芥突变体进行全面的表型和转录组分析来解决这些问题。我们证明DNA（去）甲基化过程在某些类型的先天性免疫中发挥着关键作用，并证明了获得性免疫，并且DNA（去）甲基化主要通过反式调节机制对防御相关转录组的反应性产生全局影响。
DNA methylation is antagonistically controlled by DNA methyltransferases and DNA demethylases. The level of DNA methylation controls plant gene expression on a global level. We have examined impacts of global changes in DNA methylation on the Arabidopsis immune system. A range of hypo‐methylated mutants displayed enhanced resistance to the biotrophic pathogen Hyaloperonospora arabidopsidis (Hpa), whereas two hyper‐methylated mutants were more susceptible to this pathogen. Subsequent characterization of the hypo‐methylated nrpe1 mutant, which is impaired in RNA‐directed DNA methylation, and the hyper‐methylated ros1 mutant, which is affected in DNA demethylation, revealed that their opposite resistance phenotypes are associated with changes in cell wall defence and salicylic acid (SA)‐dependent gene expression. Against infection by the necrotrophic pathogen Plectosphaerella cucumerina, nrpe1 showed enhanced susceptibility, which was associated with repressed sensitivity of jasmonic acid (JA)‐inducible gene expression. Conversely, ros1 displayed enhanced resistance to necrotrophic pathogens, which was not associated with increased responsiveness of JA‐inducible gene expression. Although nrpe1 and ros1 were unaffected in systemic acquired resistance to Hpa, they failed to develop transgenerational acquired resistance against this pathogen. Global transcriptome analysis of nrpe1 and ros1 at multiple time‐points after Hpa infection revealed that 49% of the pathogenesis‐related transcriptome is influenced by NRPE1‐ and ROS1‐controlled DNA methylation. Of the 166 defence‐related genes displaying augmented induction in nrpe1 and repressed induction in ros1, only 25 genes were associated with a nearby transposable element and NRPE1‐ and/or ROS1‐controlled DNA methylation. Accordingly, we propose that the majority of NRPE1‐ and ROS1‐dependent defence genes are regulated in trans by DNA methylation. DNA methylation has been implicated in defence priming, but it is not fully understood what types of plant immunity are influenced or to what extent DNA methylation is involved. We addressed these questions by comprehensive phenotypic and transcriptomic analysis of Arabidopsis mutants that are oppositely affected in DNA methylation. We show that DNA (de)methylation processes play critical roles in certain types of innate, and demonstrate acquired immunity and that DNA (de)methylation exerts a global influence on the responsiveness of the defence‐related transcriptome, predominantly via trans‐regulatory mechanisms.
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影响因子：
4.5
作者：
Liu ZW;Shao CR;Zhang CJ;Zhou JX;Zhang SW;Li L;Chen S;Huang HW;Cai T;He XJ
通讯作者：
He XJ
影响因子：
3.5
作者：
Lawton, K;Weymann, K;Ryals, J
通讯作者：
Ryals, J
DOI：
10.1073/pnas.1209329109
发表时间：
2012-08-07
影响因子：
11.1
作者：
Dowen, Robert H.;Pelizzola, Mattia;Ecker, Joseph R.
通讯作者：
Ecker, Joseph R.
影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
通讯作者：
Ecker, JR
影响因子：
11.6
作者：
CAO, H;BOWLING, SA;DONG, XN
通讯作者：
DONG, XN