OpenStage: a low-cost motorized microscope stage with sub-micron positioning accuracy.
OpenStage: a low-cost motorized microscope stage with sub-micron positioning accuracy.
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DOI：
10.1371/journal.pone.0088977
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Turner GC
中科院分区：
文献类型：
--
作者：
Campbell RA;Eifert RW;Turner GC
细胞内钙传感器和其他荧光团的最新进展促进了功能光学成像在生命科学中的广泛采用。自制的多光子显微镜易于制造，高度可定制，并且具有成本效益。对于许多成像应用来说，3轴电动工作台是至关重要的，但市面上可以买到的电动硬件(电动翻译机、控制器盒等)通常非常昂贵。此外，商用电机控制器上的固件不容易更改，并且通常不是在设计时考虑到显微镜平台。在这里，我们描述了一个开源的机动化解决方案，它构建简单，但比商业产品便宜得多，而且更具定制化。控制器和机动化硬件的成本不到1000美元。通过用高质量的步进电机取代直线执行器，硬件成本保持在较低水平。电子产品是由常见的业余爱好组件组装而成的，这些组件很容易操作。在这里，我们描述了系统的组装，并量化了所有三个轴的定位精度。我们获得了X/Y和Z方向的定位重复性。手持控制板允许用户在广泛的速度范围(TO)上精确地引导舞台运动，快速存储和返回到不同的位置，并执行固定大小的“跳跃”。此外，系统还可以通过PC串口进行控制。我们的“OpenStage”控制器足够灵活，只需最小的修改，就可以用来驱动其他设备，如微操作器。
Recent progress in intracellular calcium sensors and other fluorophores has promoted the widespread adoption of functional optical imaging in the life sciences. Home-built multiphoton microscopes are easy to build, highly customizable, and cost effective. For many imaging applications a 3-axis motorized stage is critical, but commercially available motorization hardware (motorized translators, controller boxes, etc) are often very expensive. Furthermore, the firmware on commercial motor controllers cannot easily be altered and is not usually designed with a microscope stage in mind. Here we describe an open-source motorization solution that is simple to construct, yet far cheaper and more customizable than commercial offerings. The cost of the controller and motorization hardware are under $1000. Hardware costs are kept low by replacing linear actuators with high quality stepper motors. Electronics are assembled from commonly available hobby components, which are easy to work with. Here we describe assembly of the system and quantify the positioning accuracy of all three axes. We obtain positioning repeatability of the order of in X/Y and in Z. A hand-held control-pad allows the user to direct stage motion precisely over a wide range of speeds ( to ), rapidly store and return to different locations, and execute “jumps” of a fixed size. In addition, the system can be controlled from a PC serial port. Our “OpenStage” controller is sufficiently flexible that it could be used to drive other devices, such as micro-manipulators, with minimal modifications.
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