Smartphone-Based Device for Colorimetric Detection of MicroRNA Biomarkers Using Nanoparticle-Based Assay.
Smartphone-Based Device for Colorimetric Detection of MicroRNA Biomarkers Using Nanoparticle-Based Assay.
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DOI：
10.3390/s21238044
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发表时间：
2021-12-01
期刊：
影响因子：
--
通讯作者：
Vo-Dinh T
中科院分区：
文献类型：
--
作者：
Krishnan T;Wang HN;Vo-Dinh T
microRNA（miRNAs）的检测正在成为用于非侵入性检测从癌症和心血管疾病到感染性疾病的各种疾病的临床重要工具。多年来，miRNA检测方案已被临床医生所接受，但它们仍然需要复杂而庞大的实验室设备和训练有素的人员来操作。现代智能手机的卓越计算能力和易用性与可现场光学检测技术相结合，可以为这些实验室系统提供有用的便携式替代方案。在此，我们提出了一种基于智能手机的设备称为Krometriks，这是能够简单和快速的比色检测microRNA（miRNA）使用基于纳米颗粒的测定的发展。该设备包括一个智能手机，一个3D打印配件，和一个定制的专用移动的应用程序。我们说明了Krometriks的效用检测一个重要的miRNA疾病生物标志物，miR-21，使用我们的小组开发的基于纳米等离子体的测定。我们发现Krometriks可以检测到低至纳摩尔浓度的miRNA，检测结果与实验室台式分光光度计相当。通过对配件设计的轻微更改，Krometriks可以与不同类型的智能手机型号和规格兼容。因此，Krometriks设备提供了一个实用的比色平台，有可能为低资源环境中的床旁和现场应用提供可访问和负担得起的miRNA诊断。
The detection of microRNAs (miRNAs) is emerging as a clinically important tool for the non-invasive detection of a wide variety of diseases ranging from cancers and cardiovascular illnesses to infectious diseases. Over the years, miRNA detection schemes have become accessible to clinicians, but they still require sophisticated and bulky laboratory equipment and trained personnel to operate. The exceptional computing ability and ease of use of modern smartphones coupled with fieldable optical detection technologies can provide a useful and portable alternative to these laboratory systems. Herein, we present the development of a smartphone-based device called Krometriks, which is capable of simple and rapid colorimetric detection of microRNA (miRNAs) using a nanoparticle-based assay. The device consists of a smartphone, a 3D printed accessory, and a custom-built dedicated mobile app. We illustrate the utility of Krometriks for the detection of an important miRNA disease biomarker, miR-21, using a nanoplasmonics-based assay developed by our group. We show that Krometriks can detect miRNA down to nanomolar concentrations with detection results comparable to a laboratory-based benchtop spectrophotometer. With slight changes to the accessory design, Krometriks can be made compatible with different types of smartphone models and specifications. Thus, the Krometriks device offers a practical colorimetric platform that has the potential to provide accessible and affordable miRNA diagnostics for point-of-care and field applications in low-resource settings.
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