KAKU4-mediated deformation of the vegetative nucleus controls its precedent migration over sperm cells in pollen tubes
KAKU4-mediated deformation of the vegetative nucleus controls its precedent migration over sperm cells in pollen tubes
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KAKU4介导的营养核变形控制其在花粉管中精子细胞上的先行迁移
DOI：
10.1101/774489
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
and Ikuko Hara-Nishimura
中科院分区：
文献类型：
--
作者：
Chieko Goto;Kentaro Tamura;Satsuki Nishimaki;Naoki Yanagisawa;Kumi Matsuura-Tokita;Tetsuya Higashiyama;Daisuke Maruyama;and Ikuko Hara-Nishimura
核纤层蛋白KAKU 4在拟南芥幼苗的核形态中具有重要的调控作用，但其生理意义尚不清楚。KAKU 4在成熟的花粉粒中有强表达，每个花粉粒都有一个营养细胞和两个精细胞。在花粉粒和伸长期的花粉管中，KAKU 4蛋白在营养核（VN）的被膜上含量较高，而在精细胞核（SCN）的被膜上含量较低。野生型花粉的VN形状不规则。然而，KAKU 4缺乏导致它变得更加球形。这些结果表明，KAKU 4的密集积累是造成VN形状不规则的原因。花粉粒萌发后，VN和SCN迁移到花粉管顶端。在野生型中，VN之前的SCN在91-93%的花粉管，而inkaku 4 mutants，VN落后的SCN在39-58%的花粉管。kaku 4花粉授粉后的竞争力低于野生型花粉，虽然它有能力受精。以上结果表明，核被蛋白KAKU 4通过控制花粉营养细胞核的形状，保证了VN和精细胞在花粉管中的有序迁移，并参与控制营养细胞核和精细胞在花粉管中的迁移顺序，影响花粉竞争受精的能力。
A putative nuclear lamina protein, KAKU4, modulates nuclear morphology inArabidopsis thalianaseedlings but its physiological significance is unknown.KAKU4was strongly expressed in mature pollen grains, each of which has a vegetative cell and two sperm cells. KAKU4 protein was highly abundant on the envelopes of vegetative nuclei (VNs) and less abundant on the envelopes of sperm cell nuclei (SCNs) in pollen grains and elongating pollen tubes. VN is irregularly shaped in wild-type pollen. However,KAKU4deficiency caused it to become more spherical. These results suggest that the dense accumulation of KAKU4 is responsible for the irregular shape of the VNs. After a pollen grain germinates, the VN and SCNs migrate to the tip of the pollen tube. In the wild type, the VN preceded the SCNs in 91–93% of the pollen tubes, whereas inkaku4mutants, the VN trailed the SCNs in 39–58% of the pollen tubes.kaku4pollen was less competitive than wild-type pollen after pollination, although it had an ability to fertilize. Taken together, our results suggest that controlling the nuclear shape in vegetative cells of pollen grains byKAKU4ensures the orderly migration of the VN and sperm cells in pollen tubes.HighlightThe nuclear envelope protein KAKU4 is involved in controlling the migration order of vegetative nuclei and sperm cells in pollen tubes, affecting the competitive ability of pollen for fertilization.
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影响因子：
64.8
作者：
Takeuchi, Hidenori;Higashiyama, Tetsuya
通讯作者：
Higashiyama, Tetsuya
影响因子：
3.7
作者：
Poulet, Axel;Probst, Aline V.;Evans, David
通讯作者：
Evans, David
影响因子：
9.2
作者：
Tamura, Kentaro;Iwabuchi, Kosei;Hara-Nishimura, Ikuko
通讯作者：
Hara-Nishimura, Ikuko
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影响因子：
5.6
作者：
Vogler F;Konrad SS;Sprunck S
通讯作者：
Sprunck S
影响因子：
--
作者：
ASTROM, H;SORRI, O;RAUDASKOSKI, M
通讯作者：
RAUDASKOSKI, M