Regional Differences in the Glycosaminoglycan Role in Porcine Scleral Hydration and Mechanical Behavior.
Regional Differences in the Glycosaminoglycan Role in Porcine Scleral Hydration and Mechanical Behavior.
复制标题
糖胺聚糖在猪巩膜水化和力学行为中作用的区域差异。
DOI：
10.1167/iovs.62.3.28
复制
发表时间：
2021-03-01
影响因子：
4.4
通讯作者：
Hatami-Marbini H
中科院分区：
文献类型：
--
作者：
Pachenari M;Hatami-Marbini H
本研究的特点的作用，糖胺聚糖（GAG）的水合作用，厚度和生物力学性能的后，前猪巩膜。取猪眼前部和后部巩膜片和巩膜条，测量其初始水化和厚度。使用前巩膜盘和后巩膜盘通过定量其GAG含量来显示GAG去除方案的功效。将条带分为PBS处理、缓冲液处理和酶处理三组，以评估不同处理程序对样品的厚度、水合作用和粘弹性的影响。通过进行单轴拉伸应力松弛实验来确定条带的机械性能。发现对照组和缓冲液组在所有测量量上均无显着差异。与来自前部区域的样品相比，来自后部区域的样品具有显著更大的GAG含量和厚度;然而，它们的水合作用无显著差异。GAG耗竭过程使前后标本的水化程度均显著降低（P < 0.05）。此外，力学测试表明，去除GAG后，前后两个样本的力学性能均有所提高（P < 0.05）。特别是，峰值应力和平衡应力显着更大的酶处理组中的条带。GAG及其与胶原网络的相互作用在定义后巩膜和前巩膜的水合和机械性质方面是重要的。
This study characterized the role of glycosaminoglycans (GAGs) in the hydration, thickness, and biomechanical properties of posterior and anterior porcine sclera. The scleral discs and strips were obtained from the anterior and posterior parts of porcine eyes, and their initial hydration and thickness were measured. The anterior and posterior scleral discs were used to show the efficacy of the GAG removal protocol by quantifying their GAG content. The strips were divided into three groups of PBS treatment, buffer treatment, and enzyme treatment in order to assess the effects of different treatment procedures on the thickness, hydration, and viscoelastic properties of the samples. The mechanical properties of the strips were determined by performing uniaxial tensile stress relaxation experiments. It was found that the control and buffer groups had insignificant differences in all measured quantities. The samples from the posterior region had a significantly larger GAG content and thickness in comparison with those from anterior region; however, there was an insignificant difference in their hydration. The GAG depletion process decreased the hydration of both anterior and posterior samples significantly (P < 0.05). Furthermore, the mechanical tests showed that the removal of GAGs resulted in stiffer mechanical behavior in both anterior and posterior samples (P < 0.05). In particular, the peak stress and equilibrium stress were significantly larger for the strips in the enzyme treatment group. GAGs and their interaction with the collagen network are important in defining the hydration and mechanical properties of both posterior and anterior sclera.
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DOI：
10.1016/j.jmbbm.2019.103562
发表时间：
2020-04-01
影响因子：
3.9
作者：
Hatami-Marbini, Hamed;Pachenari, Mohammad
通讯作者：
Pachenari, Mohammad
影响因子：
2.4
作者：
Grytz R;Girkin CA;Libertiaux V;Downs JC
通讯作者：
Downs JC
影响因子：
3.4
作者：
Elsheikh, Ahmed;Geraghty, Brendan;Rama, Paolo
通讯作者：
Rama, Paolo
影响因子：
3.4
作者：
Hatami-Marbini, Hamed;Etebu, Ebitimi
通讯作者：
Etebu, Ebitimi
影响因子：
3.4
作者：
Boubriak, OA;Urban, JPG;Bron, AJ
通讯作者：
Bron, AJ