Structural and immunochemical relatedness suggests a conserved pathogenicity motif for secondary cell wall polysaccharides in Bacillus anthracis and infection-associated Bacillus cereus.
Structural and immunochemical relatedness suggests a conserved pathogenicity motif for secondary cell wall polysaccharides in Bacillus anthracis and infection-associated Bacillus cereus.
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结构和免疫化学相关性表明，炭疽芽孢杆菌和感染相关的蜡状芽孢杆菌中二级细胞壁多糖的保守致病基序。
DOI：
10.1371/journal.pone.0183115
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Quinn CP
中科院分区：
文献类型：
--
作者：
Kamal N;Ganguly J;Saile E;Klee SR;Hoffmaster A;Carlson RW;Forsberg LS;Kannenberg EL;Quinn CP
炭疽芽孢杆菌（Ba）和人类感染相关蜡样芽孢杆菌（Bc）菌株Bc G9241和Bc 03 BB87具有二级细胞壁多糖（SCWP），其包含氨基糖基三糖重复：→4）-β-d-ManpNAc-（1→4）-β-d-GlcpNAc-（1→6）-α-d-GlcpNAc-（1→ 6），在GlcNAc残基处被α-和β-Galp取代。在Bc G9241和Bc 03 BB87中，额外的α-Galp连接到ManNAc的O-3。使用核磁共振光谱法、质谱法和免疫化学方法，我们将这些结构与从喀麦隆（BC CA）和科特迪瓦（BC CI）表现出“炭疽样”症状的类人猿中分离的BC生物变种炭疽菌株的SCWP进行了比较。Bc CA/CI的SCWP含有Ba中发现的相同的HexNAc三糖骨架和Gal修饰，以及先前仅在Bc G9241/03 BB87中的ManNAc残基处观察到的α-Gal-（1→3）取代。有趣的是，类人猿来源的菌株在某些ManNAc残基上显示出独特的α-Gal-（1→3）-α-Gal-（1→3）二糖取代，这在任何先前检测的Ba或Bc菌株中均未发现。用特异性多克隆抗Ba SCWP抗血清进行的免疫分析显示了反应性等级：与Ba 7702和Ba Sterne 34 F2以及Bc G9241和Bc 03 BB87的SCWP具有高反应性;与Bc CI/CA的SCWP具有中等反应性;与来自结构上不同的Ba CDC 684的SCWP的反应性低（产生缺乏所有Gal取代的SCWP的独特菌株）和非感染相关的Bc ATCC 10987和Bc 14579 SCWP。Ba特异性单克隆抗体EAII-6 G6 -2-3显示与Ba 7702/34 F2相比，对Bc G9241和Bc 03 BB87 SCWP的反应性降低10-20倍，并且对来自Bc Cl、Bc CA、Ba CDC 684和非感染相关Bc菌株的SCWP的反应性低/不可检测。我们的数据表明，HexNAc基序是保守的感染相关的Ba和Bc分离株（无论人类或类人猿的起源），和Gal取代的数量，位置和结构赋予独特的抗原特性。该结构基序的保守性可能为致病性Bc菌株的检测开辟一条新的诊断途径。
Bacillus anthracis (Ba) and human infection-associated Bacillus cereus (Bc) strains Bc G9241 and Bc 03BB87 have secondary cell wall polysaccharides (SCWPs) comprising an aminoglycosyl trisaccharide repeat: →4)-β-d-ManpNAc-(1→4)-β-d-GlcpNAc-(1→6)-α-d-GlcpNAc-(1→, substituted at GlcNAc residues with both α- and β-Galp. In Bc G9241 and Bc 03BB87, an additional α-Galp is attached to O-3 of ManNAc. Using NMR spectroscopy, mass spectrometry and immunochemical methods, we compared these structures to SCWPs from Bc biovar anthracis strains isolated from great apes displaying “anthrax-like” symptoms in Cameroon (Bc CA) and Côte d’Ivoire (Bc CI). The SCWPs of Bc CA/CI contained the identical HexNAc trisaccharide backbone and Gal modifications found in Ba, together with the α-Gal-(1→3) substitution observed previously at ManNAc residues only in Bc G9241/03BB87. Interestingly, the great ape derived strains displayed a unique α-Gal-(1→3)-α-Gal-(1→3) disaccharide substitution at some ManNAc residues, a modification not found in any previously examined Ba or Bc strain. Immuno-analysis with specific polyclonal anti-Ba SCWP antiserum demonstrated a reactivity hierarchy: high reactivity with SCWPs from Ba 7702 and Ba Sterne 34F2, and Bc G9241 and Bc 03BB87; intermediate reactivity with SCWPs from Bc CI/CA; and low reactivity with the SCWPs from structurally distinct Ba CDC684 (a unique strain producing an SCWP lacking all Gal substitutions) and non-infection-associated Bc ATCC10987 and Bc 14579 SCWPs. Ba-specific monoclonal antibody EAII-6G6-2-3 demonstrated a 10–20 fold reduced reactivity to Bc G9241 and Bc 03BB87 SCWPs compared to Ba 7702/34F2, and low/undetectable reactivity to SCWPs from Bc CI, Bc CA, Ba CDC684, and non-infection-associated Bc strains. Our data indicate that the HexNAc motif is conserved among infection-associated Ba and Bc isolates (regardless of human or great ape origin), and that the number, positions and structures of Gal substitutions confer unique antigenic properties. The conservation of this structural motif could open a new diagnostic route in detection of pathogenic Bc strains.
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影响因子：
3.6
作者：
CATALDI, A;LABRUYERE, E;MOCK, M
通讯作者：
MOCK, M
影响因子：
4.8
作者：
Choudhury, Biswa;Leoff, Christine;Carlson, Russell W.
通讯作者：
Carlson, Russell W.
影响因子：
4.1
作者：
Gevorkian, G;Segura, E;López-Marín, LM
通讯作者：
López-Marín, LM
影响因子：
9.4
作者：
Hoffmaster, Alex R.;Hill, Karen K.;Jackson, Paul J.
通讯作者：
Jackson, Paul J.
DOI：
10.1073/pnas.0402414101
发表时间：
2004-06-01
影响因子：
11.1
作者：
Hoffmaster, AR;Ravel, J;Fraser, CM
通讯作者：
Fraser, CM