Cells and tissue interactions with glycated collagen and their relevance to delayed diabetic wound healing.
Cells and tissue interactions with glycated collagen and their relevance to delayed diabetic wound healing.
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DOI：
10.1016/j.biomaterials.2008.11.038
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发表时间：
2009-03
期刊：
影响因子：
14
通讯作者：
Chen, Weiliam
中科院分区：
文献类型：
--
作者：
Liao, Huijuan;Zakhaleva, Julia;Chen, Weiliam
晚期糖基化终末产物 (AGE) 的真皮积累越来越多地被认为是糖尿病伤口愈合延迟的根本原因。设计充分模拟糖化组织的体外模型将有助于研究糖化机制，同时探索治疗糖尿病慢性伤口的新方法或改进现有疗法。胶原基质被人工糖化，并通过免疫染色证明了 AGE 的存在。糖化后，胶原基质的机械特性及其与成纤维细胞的相互作用（形态、附着、增殖和迁移）都发生了改变，此外，有证据表明细胞外基质（ECM）重塑受损以及细胞诱导的材料收缩受到抑制。糖化胶原基质中成纤维细胞的肌动蛋白细胞骨架被重组。植入糖化胶原基质的小鼠体内全层真皮伤口模型显示伤口愈合反应延迟。因此，糖化胶原基质是模拟糖化组织的适当体外模型，并且可以作为研究糖化机制的简便实验工具，同时探索治疗糖尿病伤口的新方法或改进现有疗法。
Dermal accumulation of advanced glycation end products (AGEs) has increasingly been implicated as the underlying cause of delayed diabetic wound healing. Devising an in vitro model to adequately mimic glycated tissues will facilitate investigation into the mechanism of glycation in conjunction with exploration of new approaches or improvement of current therapies for treating diabetic chronic wounds. Collagen matrices were artificially glycated and the presence of AGEs was demonstrated by immunostaining. Both the mechanical properties of the collagen matrices and their interactions with fibroblasts (morphology, attachment, proliferation, and migration) were altered after glycation, moreover, there was evidence of impairment on extracellular matrix (ECM) remodeling as well as inhibition of cell-induced material contraction. The actin cytoskeletons of the fibroblasts residing in the glycated collagen matrices were reorganized. In vivo mice full-thickness dermal wound models implanted with glycated collagen matrices showed delayed wound healing response. Thus, the glycated collagen matrix is an adequate in vitro model to mimic glycated tissues and could serve as a facile experimental tool to investigate the mechanism of glycation in conjunction with exploration of new approaches or improvement of current therapies for treating diabetic wounds.
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影响因子：
3.7
作者：
GULLBERG, D;TINGSTROM, A;RUBIN, K
通讯作者：
RUBIN, K
影响因子：
14
作者：
Ng, KW;Khor, HL;Hutmacher, DW
通讯作者：
Hutmacher, DW
影响因子：
4.1
作者：
Ahmed, MU;Frye, EB;Baynes, JW
通讯作者：
Baynes, JW
DOI：
10.1073/pnas.76.3.1274
发表时间：
1979-01-01
影响因子：
11.1
作者：
BELL, E;IVARSSON, B;MERRILL, C
通讯作者：
MERRILL, C
影响因子：
4.8
作者：
Kislinger, T;Fu, CF;Schmidt, AM
通讯作者：
Schmidt, AM