microRNA-124 regulates Notch and NeuroD1 to mediate transition states of neuronal development.
microRNA-124 regulates Notch and NeuroD1 to mediate transition states of neuronal development.
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DOI：
10.1002/dneu.22902
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发表时间：
2023-01
影响因子：
3
通讯作者：
中科院分区：
文献类型：
--
作者：
MicroRNA 通过破坏动物细胞中靶 mRNA 的稳定性和/或抑制翻译来调节基因表达。在单个系统内发育过程中神经元的规范、分化和成熟过程中机械剖析 miR-124 功能的能力尚未实现。使用海胆胚胎，我们利用了胚胎的可操作性及其有据可查的基因调控网络（GRN）。我们将 NeuroD1 作为海胆神经元 GRN 的一部分，并确定 miR-124 抑制会导致异常的肠道收缩、游泳速度和神经元发育。抑制 miR-124 导致表达与祖神经元相关的转录因子 (TF) 的细胞数量增加，同时成熟和功能神经元的减少。结果显示，在早期囊胚/原肠胚阶段，miR-124 在神经元规范和分化过程中调节未定义的因素。在原肠胚晚期/幼虫阶段，miR-124 在神经元分化和成熟之间的过渡期间调节 Notch 和 NeuroD1。总体而言，我们改进了神经元 GRN，并确定 miR-124 在调节神经元发育的各种转变中发挥着重要作用。
MicroRNAs regulate gene expression by destabilizing target mRNA and/or inhibiting translation in animal cells. The ability to mechanistically dissect miR-124′s function during specification, differentiation, and maturation of neurons during development within a single system has not been accomplished. Using the sea urchin embryo, we take advantage of the manipulability of the embryo and its well-documented gene regulatory networks (GRNs). We incorporated NeuroD1 as part of the sea urchin neuronal GRN and determined that miR-124 inhibition resulted in aberrant gut contractions, swimming velocity, and neuronal development. Inhibition of miR-124 resulted in an increased number of cells expressing transcription factors (TFs) associated with progenitor neurons and a concurrent decrease of mature and functional neurons. Results revealed that in the early blastula/gastrula stages, miR-124 regulates undefined factors during neuronal specification and differentiation. In the late gastrula/larval stages, miR-124 regulates Notch and NeuroD1 during the transition between neuronal differentiation and maturation. Overall, we have improved the neuronal GRN and identified miR-124 to play a prolific role in regulating various transitions of neuronal development.
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影响因子：
2.7
作者：
Gustafson EA;Yajima M;Juliano CE;Wessel GM
通讯作者：
Wessel GM
影响因子：
4.6
作者：
Braun, MM;Etheridge, A;Roelink, H
通讯作者：
Roelink, H
影响因子：
23.9
作者：
Ambasudhan R;Talantova M;Coleman R;Yuan X;Zhu S;Lipton SA;Ding S
通讯作者：
Ding S
影响因子：
2.5
作者：
KIMMEL, CB;BALLARD, WW;SCHILLING, TF
通讯作者：
SCHILLING, TF
影响因子：
4.6
作者：
Krupke, Oliver A.;Burke, Robert D.
通讯作者：
Burke, Robert D.