Post-translational regulation by gustavus contributes to selective Vasa protein accumulation in multipotent cells during embryogenesis.
Post-translational regulation by gustavus contributes to selective Vasa protein accumulation in multipotent cells during embryogenesis.
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DOI：
10.1016/j.ydbio.2010.10.031
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发表时间：
2011-01-15
影响因子：
2.7
通讯作者：
Wessel GM
中科院分区：
文献类型：
--
作者：
Gustafson EA;Yajima M;Juliano CE;Wessel GM
Vasa是一种广泛保守的DEAD-box RNA解旋酶，与种系发育有关，在许多动物的多能细胞中表达。在紫癜圆心海胆的胚胎发育过程中，尽管Vasa转录本分布均匀，但Vasa蛋白在小微粒富集。本研究表明Vasa编码区有足够的选择性富集，并发现B30.2/SPRY和SOCS盒域基因gustavus对这一现象有贡献。体外结合分析表明，Gustavus独立结合Vasa蛋白的n端和DEAD-box部分。Gustavus蛋白的敲低会降低Vasa蛋白的丰度及其在小微粒中积累的倾向，而Gustavus的Vasa相互作用域的过表达（GusΔSOCS）会导致整个胚胎中Vasa蛋白的积累。我们认为Gustavus在胚胎发育过程中对Vasa蛋白积累具有保守的、积极的调节作用。
Vasa is a broadly conserved DEAD-box RNA helicase associated with germ line development and is expressed in multipotent cells in many animals. During embryonic development of the sea urchin Strongylocentrotus purpuratus, Vasa protein is enriched in the small micromeres despite a uniform distribution of vasa transcript. Here we show that the Vasa coding region is sufficient for its selective enrichment and find that gustavus, the B30.2/SPRY and SOCS box domain gene, contributes to this phenomenon. In vitro binding analyses show that Gustavus binds the N-terminal and DEAD-box portions of Vasa protein independently. A knockdown of Gustavus protein reduces both Vasa protein abundance and its propensity for accumulation in the small micromeres, whereas overexpression of the Vasa-interacting domain of Gustavus (GusΔSOCS) results in Vasa protein accumulation throughout the embryo. We propose that Gustavus has a conserved, positive regulatory role in Vasa protein accumulation during embryonic development.
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影响因子：
9.2
作者：
Liu, NK;Dansereau, DA;Lasko, P
通讯作者：
Lasko, P
Pfam：氏族、网络工具和服务。
影响因子：
14.9
作者：
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作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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