Genome sequencing and comparative genomic analysis of highly and weakly aggressive strains of Sclerotium rolfsii, the causal agent of peanut stem rot.
Genome sequencing and comparative genomic analysis of highly and weakly aggressive strains of Sclerotium rolfsii, the causal agent of peanut stem rot.
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花生茎腐病菌强、弱致病性白绢病菌株的基因组测序及比较基因组分析。
DOI：
10.1186/s12864-021-07534-0
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发表时间：
2021-04-16
期刊：
影响因子：
4.4
通讯作者：
Liao B
中科院分区：
文献类型：
--
作者：
Yan L;Wang Z;Song W;Fan P;Kang Y;Lei Y;Wan L;Huai D;Chen Y;Wang X;Sudini H;Liao B
花生茎腐病是一种重要的花生土传病害。罗氏菌是一种具有广泛寄主范围和世界分布的坏死性植物病原真菌。它可以感染花生茎、根、茎钉和豆荚，导致各种产量损失。通过人工接种试验，在中国不同省份花生中采集的罗尔夫氏沙门氏菌GP3和ZY菌株对花生植株的侵袭性存在显著差异。本研究对这两种不同菌株进行从头基因组测序，旨在揭示其侵袭性差异的基因组基础。罗氏核孢菌GP3和ZY菌株对花生的侵袭性分别较弱和较高，在实验室中表现出相似的生长速度和草酸产量。采用Pacbio长读技术对S. rolfsii菌株GP3和ZY的基因组进行测序，结果显示菌株基因组长度分别为70.51 Mb和70.61 Mb， contigs为27和23，分别编码17097和16743个基因模型。比较基因组分析显示，GP3和ZY的致病性相关基因库可能与侵袭性有关。GP3和ZY中分别存在58个和45个独特的病原-宿主相互作用基因。ZY菌株分泌组中糖活性酶（CAZymes）比GP3多，特别是糖苷水解酶家族（GH）、糖酯酶家族（CBM）和多糖裂解酶家族（PL）。GP3和ZY也有不同的候选效应物和推测的次生代谢产物合成基因簇。这些结果表明，两菌株在PHI、分泌的CAZymes、效应物和次生代谢物方面的差异可能是造成其侵袭性差异的重要原因。这些数据为进一步了解S. rolfsii基因组提供了依据。基因组比较提供了罗弗氏菌株侵袭性差异的线索。在线版本包含补充材料，可在10.1186/s12864-021-07534-0获得。
Stem rot caused by Sclerotium rolfsii is a very important soil-borne disease of peanut. S. rolfsii is a necrotrophic plant pathogenic fungus with an extensive host range and worldwide distribution. It can infect peanut stems, roots, pegs and pods, leading to varied yield losses. S. rolfsii strains GP3 and ZY collected from peanut in different provinces of China exhibited a significant difference in aggressiveness on peanut plants by artificial inoculation test. In this study, de-novo genome sequencing of these two distinct strains was performed aiming to reveal the genomic basis of difference in aggressiveness. Scleotium rolfsii strains GP3 and ZY, with weak and high aggressiveness on peanut plants, exhibited similar growth rate and oxalic acid production in laboratory. The genomes of S. rolfsii strains GP3 and ZY were sequenced by Pacbio long read technology and exhibited 70.51 Mb and 70.61 Mb, with contigs of 27 and 23, and encoded 17,097 and 16,743 gene models, respectively. Comparative genomic analysis revealed that the pathogenicity-related gene repertoires, which might be associated with aggressiveness, differed between GP3 and ZY. There were 58 and 45 unique pathogen-host interaction (PHI) genes in GP3 and ZY, respectively. The ZY strain had more carbohydrate-active enzymes (CAZymes) in its secretome than GP3, especially in the glycoside hydrolase family (GH), the carbohydrate esterase family (CBM), and the polysaccharide lyase family (PL). GP3 and ZY also had different effector candidates and putative secondary metabolite synthetic gene clusters. These results indicated that differences in PHI, secreted CAZymes, effectors and secondary metabolites may play important roles in aggressive difference between these two strains. The data provided a further understanding of the S. rolfsii genome. Genomic comparison provided clues to the difference in aggressiveness of S. rolfsii strains. The online version contains supplementary material available at 10.1186/s12864-021-07534-0.
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影响因子：
3.3
作者：
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影响因子：
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