Evaluation of dsRNA delivery methods for targeting macrophage migration inhibitory factor MIF in RNAi-based aphid control
Evaluation of dsRNA delivery methods for targeting macrophage migration inhibitory factor MIF in RNAi-based aphid control
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基于 RNAi 的蚜虫控制中靶向巨噬细胞迁移抑制因子 MIF 的 dsRNA 递送方法的评估
DOI：
10.1007/s41348-021-00464-9
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发表时间：
2021
影响因子：
2
通讯作者：
Imani J
中科院分区：
文献类型：
--
作者：
Ladera-Carmona MJ;Poranen MM;van Bel Aart J.E;Kogel K-H;Imani J
巨噬细胞移动抑制因子（MIFs）是一种多功能蛋白质，调节哺乳动物的主要过程，包括激活先天性免疫反应。在无脊椎动物中，当由寄生生物（如蚜虫）分泌时，MIF蛋白参与调节宿主免疫应答。在这项研究中，我们评估的可能性，使用MIF基因作为目标的RNA干扰（RNAi）为基础的控制麦长管蚜（Sa）在大麦（大麦）。当以含有靶向三个MIF基因SaMIF 1、SaMIF 2和SaMIF 3序列的双链RNA（SaMIF-dsRNA）的人工饲料喂养蚜虫时，与以含有对照dsRNA（GFP-dsRNA）的人工饲料喂养蚜虫相比，它们表现出更高的死亡率，并且这些死亡率与MIF转录水平降低相关。不同取食策略的比较表明，当它们从喷洒了裸SaMIF-dsRNA的大麦幼苗中取食时，它们的存活率没有改变，这表明它们不能有效地从这些植物的筛管中摄取dsRNA。此外，蚜虫的生存也没有受到影响，当取食的叶片与dsRNA通过大麦叶片的基部切割端提供。与这一发现一致，筛管特异性YFP标记的拟南芥报告基因系的使用证实了荧光21 nt dsRNA γ 3，当通过叶柄或喷雾供应时，与木质部结构共定位，但不与韧皮部组织共定位。我们的研究结果表明，MIF基因是一个潜在的目标，昆虫控制，也意味着裸dsRNA的应用于植物蚜虫控制是无效的。因此，应进一步努力开发有效的dsRNA制剂。
Macrophage migration inhibitory factors (MIFs) are multifunctional proteins regulating major processes in mammals, including activation of innate immune responses. In invertebrates, MIF proteins participate in the modulation of host immune responses when secreted by parasitic organisms, such as aphids. In this study, we assessed the possibility to useMIFgenes as targets for RNA interference (RNAi)-based control of the grain aphidSitobion avenae(Sa) on barley (Hordeum vulgare). When nymphs were fed on artificial diet containing double-stranded (ds)RNAs (SaMIF-dsRNAs) that target sequences of the threeMIFgenesSaMIF1,SaMIF2andSaMIF3, they showed higher mortality rates and these rates correlated with reducedMIFtranscript levels as compared to the aphids feeding on artificial diet containing a control dsRNA (GFP-dsRNA). Comparison of different feeding strategies showed that nymphs’ survival was not altered when they fed from barley seedlings sprayed with nakedSaMIF-dsRNAs, suggesting they did not effectively take up dsRNA from the sieve tubes of these plants. Furthermore, aphids’ survival was also not affected when the nymphs fed on leaves supplied with dsRNA via basal cut ends of barley leaves. Consistent with this finding, the use of sieve tube-specific YFP-labeledArabidopsisreporter lines confirmed that fluorescent 21 nt dsRNACy3, when supplied via petioles or spraying, co-localized with xylem structures, but not with phloem tissue. Our results suggest thatMIFgenes are a potential target for insect control and also imply that application of naked dsRNA to plants for aphid control is inefficient. More efforts should be put into the development of effective dsRNA formulations.
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影响因子：
7.4
作者：
Schwartz, Steven H.;Hendrix, Bill;Zheng, Wei
通讯作者：
Zheng, Wei
影响因子：
3.5
作者：
C. Michelet;E. Danchin;M. Jaouannet;J. Bernhagen;R. Panstruga;K. Kogel;H. Keller;C. Coustau
通讯作者：
C. Michelet;E. Danchin;M. Jaouannet;J. Bernhagen;R. Panstruga;K. Kogel;H. Keller;C. Coustau
影响因子：
3.4
作者：
Fernando Aranguren, Luis;Salazar, Marcela;Lightner, Donald
通讯作者：
Lightner, Donald
影响因子：
7.4
作者：
Wan, Juan;Cabanillas, Daniel Garcia;Laliberte, Jean-Francois
通讯作者：
Laliberte, Jean-Francois
影响因子：
5.6
作者：
Dalakouras A;Jarausch W;Buchholz G;Bassler A;Braun M;Manthey T;Krczal G;Wassenegger M
通讯作者：
Wassenegger M