The kinetics of humoral response and its relationship with the disease severity in COVID-19.
The kinetics of humoral response and its relationship with the disease severity in COVID-19.
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DOI：
10.1038/s42003-020-01526-8
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发表时间：
2020-12-11
影响因子：
5.9
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Ren L;Zhang L;Chang D;Wang J;Hu Y;Chen H;Guo L;Wu C;Wang C;Wang Y;Wang Y;Wang G;Yang S;Dela Cruz CS;Sharma L;Wang L;Zhang D;Wang J
2019冠状病毒病（COVID-19）已导致全球大流行。在这里，我们通过测量针对核壳和刺突蛋白的免疫球蛋白（IG）A、IgM和IgG，沿着针对受体结合结构域（RBD）的IgM和IgG抗体，分析了针对严重急性呼吸系统综合征冠状病毒2型（SARS-CoV-2）的体液反应。刺突蛋白和总中和抗体（NAbs）。我们检测了从176名COVID-19患者中收集的279份血浆样本，这些患者在疾病的不同阶段就诊和入组。优化血浆稀释度，并基于数据，使用单次血浆稀释。测量IG水平在450 nm处的平均吸光度，并使用几何平均滴度测量NAb。我们证明，更严重的情况下，有一个迟发性的体液反应相比，轻度/中度感染。在COVID-19患者的病程中，所有抗体滴度持续上升。然而，这些水平大多与疾病的严重程度无关。NAb的出现时间和滴度与抗刺突蛋白抗体呈显著正相关。我们的研究结果表明，抗体反应的迟发性是疾病严重程度的一个风险因素，然而，抗体滴度在预测COVID-19疾病严重程度方面的作用有限。Lili Ren等人研究了176名COVID-19患者的抗体反应，这些患者表现出疾病的不同阶段。这项研究表明，抗体反应的迟发性是疾病严重程度的一个风险因素，抗体滴度在预测COVID-19疾病严重程度方面的作用有限。
Coronavirus Disease 2019 (COVID-19) has caused a global pandemic. Here we profiled the humoral response against Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by measuring immunoglobulin (Ig) A, IgM, and IgG against nucleocapsid and spike proteins, along with IgM and IgG antibodies against receptor-binding domain (RBD) of the spike protein and total neutralizing antibodies (NAbs). We tested 279 plasma samples collected from 176 COVID-19 patients who presented and enrolled at different stages of their disease. Plasma dilutions were optimized and based on the data, a single dilution of plasma was used. The mean absorbance at 450 nm was measured for Ig levels and NAbs were measured using geometric mean titers. We demonstrate that more severe cases have a late-onset in the humoral response compared to mild/moderate infections. All the antibody titers continue to rise in patients with COVID-19 over the disease course. However, these levels are mostly unrelated to disease severity. The appearance time and titers of NAbs showed a significant positive correlation to the antibodies against spike protein. Our results suggest the late onset of antibody response as a risk factor for disease severity, however, there is a limited role of antibody titers in predicting disease severity of COVID-19. Lili Ren et al. investigate antibody response from 176 COVID-19 patients who presented different stages of their disease. This study suggests the late onset of antibody response as a risk factor for disease severity and a limited role of antibody titers in predicting the disease severity of COVID-19.
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影响因子：
11.8
作者：
Guo L;Zhang X;Ren L;Yu X;Chen L;Zhou H;Gao X;Teng Z;Li J;Hu J;Wu C;Xiao X;Zhu Y;Wang Q;Pang X;Jin Q;Wu F;Wang J
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