The RepA_N replicons of Gram-positive bacteria: a family of broadly distributed but narrow host range plasmids.
The RepA_N replicons of Gram-positive bacteria: a family of broadly distributed but narrow host range plasmids.
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DOI：
10.1016/j.plasmid.2008.11.004
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发表时间：
2009-03
期刊：
影响因子：
2.6
通讯作者：
Francia MV
中科院分区：
文献类型：
--
作者：
Weaver KE;Kwong SM;Firth N;Francia MV
粪肠球菌的信息素应答接合质粒和金黄色葡萄球菌的多重抗性质粒pSK 1和pSK 41是革兰氏阳性菌天然的研究最好的质粒。虽然这些质粒似乎在很大程度上限于它们的天然宿主，但它们的复制起始蛋白的蛋白质序列比较表明它们明显相关。同源性搜索表明，这些复制子是一个大家族的质粒和一些噬菌体，广泛存在于低G+C革兰氏阳性菌的代表。我们建议将这个家族命名为RepA_N家族的复制子后，注释的保守结构域的起始蛋白包含。详细的序列比较表明，起始蛋白的复制基因在很大程度上是一致的，与主机，这表明复制子已经进化沿着与他们目前的主机和属间转移已经罕见。然而，相关的蛋白质被确定在染色体区域轴承ICE元件的特征指示，这些蛋白质的同源性显示更频繁的属间转移的证据。与RepA_N复制子相关的稳定性决定子的比较表明，它们具有在其他质粒家族中观察到的模块化进化。
The pheromone-responsive conjugative plasmids of Enterococcus faecalis and the multi-resistance plasmids pSK1 and pSK41 of Staphylococcus aureus are among the best studied plasmids native to Gram-positive bacteria. Although these plasmids seem largely restricted to their native hosts, protein sequence comparison of their replication initiator proteins indicates that they are clearly related. Homology searches indicate that these replicons are representatives of a large family of plasmids and a few phage that are widespread among the low G+C Gram-positive bacteria. We propose to name this family the RepA_N family of replicons after the annotated conserved domain that the initiator protein contains. Detailed sequence comparisons indicate that the initiator protein phylogeny is largely congruent with that of the host, suggesting that the replicons have evolved along with their current hosts and that intergeneric transfer has been rare. However, related proteins were identified on chromosomal regions bearing characteristics indicative of ICE elements, and the phylogeny of these proteins displayed evidence of more frequent intergeneric transfer. Comparison of stability determinants associated with the RepA_N replicons suggests that they have a modular evolution as has been observed in other plasmid families.
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影响因子：
3.7
作者：
Chen C;Tang J;Dong W;Wang C;Feng Y;Wang J;Zheng F;Pan X;Liu D;Li M;Song Y;Zhu X;Sun H;Feng T;Guo Z;Ju A;Ge J;Dong Y;Sun W;Jiang Y;Wang J;Yan J;Yang H;Wang X;Gao GF;Yang R;Wang J;Yu J
通讯作者：
Yu J
影响因子：
3.6
作者：
De Boever, EH;Clewell, DB;Fraser, CM
通讯作者：
Fraser, CM
影响因子：
3.5
作者：
Gering, M;Gotz, F;Bruckner, R
通讯作者：
Bruckner, R
DOI：
10.1099/00221287-146-6-1481
发表时间：
2000-06-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
Garnier, F;Taourit, S;Galimand, M
通讯作者：
Galimand, M
DOI：
10.1073/pnas.152298499
发表时间：
2002-07-23
影响因子：
11.1
作者：
Beres, SB;Sylva, GL;Musser, JM
通讯作者：
Musser, JM