A high-resolution interactive atlas of the human brainstem using magnetic resonance imaging.
A high-resolution interactive atlas of the human brainstem using magnetic resonance imaging.
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DOI：
10.1016/j.neuroimage.2021.118135
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发表时间：
2021-08-15
期刊：
影响因子：
5.7
通讯作者：
White LE
中科院分区：
文献类型：
--
作者：
Adil SM;Calabrese E;Charalambous LT;Cook JJ;Rahimpour S;Atik AF;Cofer GP;Parente BA;Johnson GA;Lad SP;White LE
人类脑干的传统图谱受到组织学的不灵活、采样稀疏、二维性质或传统磁共振成像 (MRI) 的低空间分辨率的限制。尸检高分辨率 MRI 规避了与这两种方式相关的挑战。一名 65 岁男性在死亡 24 小时内摘除大脑后，准备了一个从头侧间脑延伸到尾髓质的单个人类脑干样本，用于成像。将样本用福尔马林固定两周，然后再水化并放入定制的 MRI 兼容管中并浸入液体碳氟化合物中。 MRI 在具有 120 个独特扩散方向的 7-Tesla 扫描仪中进行。解剖图像和扩散图像的采集时间分别为 14 小时和 208 小时。分段是手动进行的。确定性纤维束成像是使用策略性选择的感兴趣和回避区域来完成的，并使用人类神经解剖学的专业知识进行手动编辑。解剖图像和扩散图像分别以 50 μm 和 200 μm 的各向同性分辨率渲染。 90 种不同的结构被分割和标记，11 种不同的纤维束通过纤维束成像呈现。完整的图集可在线获取以供交互式使用：https://www.civmvoxport.vm.duke.edu/voxbase/login.php?return_url=%2Fvoxbase%2F。该图集提供了多个对比数据集和选定的束重建，具有前所未有的分辨率，用于人类脑干的 MR 成像。它可以立即应用于神经解剖学教育，并有可能为未来的神经解剖学研究应用提供服务，并通过进入人类脑干的“安全”区域增强神经外科规划。
Conventional atlases of the human brainstem are limited by the inflexible, sparsely-sampled, two-dimensional nature of histology, or the low spatial resolution of conventional magnetic resonance imaging (MRI). Postmortem high-resolution MRI circumvents the challenges associated with both modalities. A single human brainstem specimen extending from the rostral diencephalon through the caudal medulla was prepared for imaging after the brain was removed from a 65-year-old male within 24 h of death. The specimen was formalin-fixed for two weeks, then rehydrated and placed in a custom-made MRI compatible tube and immersed in liquid fluorocarbon. MRI was performed in a 7-Tesla scanner with 120 unique diffusion directions. Acquisition time for anatomic and diffusion images were 14 h and 208 h, respectively. Segmentation was performed manually. Deterministic fiber tractography was done using strategically chosen regions of interest and avoidance, with manual editing using expert knowledge of human neuroanatomy. Anatomic and diffusion images were rendered with isotropic resolutions of 50 μm and 200 μm, respectively. Ninety different structures were segmented and labeled, and 11 different fiber bundles were rendered with tractography. The complete atlas is available online for interactive use at https://www.civmvoxport.vm.duke.edu/voxbase/login.php?return_url=%2Fvoxbase%2F. This atlas presents multiple contrasting datasets and selected tract reconstruction with unprecedented resolution for MR imaging of the human brainstem. There are immediate applications in neuroanatomical education, with the potential to serve future applications for neuroanatomical research and enhanced neurosurgical planning through “safe” zones of entry into the human brainstem.
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影响因子：
4.8
作者：
Calabrese E;Hickey P;Hulette C;Zhang J;Parente B;Lad SP;Johnson GA
通讯作者：
Johnson GA
DOI：
10.1007/s10334-016-0546-3
发表时间：
2016-06-01
影响因子：
2.3
作者：
Bianciardi, Marta;Toschi, Nicola;Wald, Lawrence L.
通讯作者：
Wald, Lawrence L.
影响因子：
2.9
作者：
Calabrese E
通讯作者：
Calabrese E
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
5.7
作者：
Fonov V;Evans AC;Botteron K;Almli CR;McKinstry RC;Collins DL;Brain Development Cooperative Group
通讯作者：
Brain Development Cooperative Group