Colonic Anastomosis with a Doxycycline-Coated Stent: An Experimental Study in a Porcine Model
Colonic Anastomosis with a Doxycycline-Coated Stent: An Experimental Study in a Porcine Model
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多西环素涂层支架结肠吻合术：猪模型的实验研究
DOI：
10.1159/000358811
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发表时间：
2014-04
影响因子：
2.7
通讯作者：
Cai, XiuJun
中科院分区：
文献类型：
--
作者：
Wang, YiFan;Cai, XiaoYan;Mei, JinHua;Liu, Kun;Cai, XiuJun
背景：基质金属蛋白酶（MMPs）被认为是预防结肠吻合口瘘的潜在药物靶点。本研究设计了一种应用MMP抑制剂强力霉素涂层支架进行结肠吻合的方法，并通过与传统方法或无强力霉素支架的方法进行比较，评价了该方法的安全性、可行性以及局部调节MMP的效果。研究方法：将48只猪随机分为多西环素涂层支架吻合组（DSA）、无多西环素支架吻合组（SA）和常规吻合组（CA）。每组中，根据术后观察时间（3、7、14和30天）将猪再分为4个亚组。观察吻合口愈合情况及MMP-2/9的表达。结果：DSA组无吻合口瘘、狭窄及坏死发生。DSA组和SA组的爆破压差异无统计学意义。术后第3天和第7天，DSA组MMP-2的相对表达明显低于SA组。各组间羟脯氨酸含量、微血管密度及TGF-β1水平无显著性差异。结论：该方法具有局部抑制MMPs的优点，是一种安全可行的结肠吻合方法。
Background: Matrix metalloproteinases (MMPs) are supposed to be potential drug targets to prevent leakage after colonic anastomosis. A method of colonic anastomosis by using a stent coated with doxycycline, a MMP inhibitor, was developed and its safety and feasibility, as well as the effect of locally regulating MMPs, were evaluated by comparing with the conventional method or the method with a doxycycline-free stent. Methods: 48 pigs were assigned randomly to doxycycline-coated stent anastomosis group (DSA), doxycycline-free stent anastomosis group (SA), or conventional anastomosis group (CA). In each group, pigs were subdivided into four subgroups according to postoperative observation time (3, 7, 14, and 30 days). Healing of anastomosis and expressions of MMP-2/9 were evaluated. Results: No anastomotic leakage, stricture or necrosis was observed in the DSA group. No significant difference of bursting pressure was found between the DSA group and SA group. Relative expression of MMP-2 in the DSA group was significantly lower than in the SA group on postoperative days 3 and 7. No significant differences of hydroxyproline content, microvessel density and TGF-β1 level were found in these groups. Conclusion: These results suggested this method was feasible and safe for colonic anastomosis with the advantage of locally inhibiting MMPs.
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影响因子：
2.9
作者：
Jianfei Wang;Haiyan Jiao;T. Stewart;H. Shankowsky;P. Scott;E. Tredget
通讯作者：
Jianfei Wang;Haiyan Jiao;T. Stewart;H. Shankowsky;P. Scott;E. Tredget
影响因子：
3.7
作者：
B. Pasternak;Anna Missios;A. Askendal;P. Tengvall;P. Aspenberg
通讯作者：
B. Pasternak;Anna Missios;A. Askendal;P. Tengvall;P. Aspenberg
影响因子：
9.6
作者：
I. Syk;Magnus S. Ågren;D. Adawi;Bengt Jeppsson
通讯作者：
I. Syk;Magnus S. Ågren;D. Adawi;Bengt Jeppsson
DOI：
10.1016/s0001-4001(98)80129-3
发表时间：
1998-06
期刊：
Chirurgie
影响因子：
--
作者：
M. Huguier
通讯作者：
M. Huguier
影响因子：
2.8
作者：
Thornton, Michael;Joshi, Heman;Rooney, Paul
通讯作者：
Rooney, Paul